Human Fibrinogen ELISA Kit人纤维蛋白原ELISA试剂盒_详细资料

Description	In this assay the FIBRINOGEN present in samples reacts with the anti-FIBRINOGEN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Intended Use	The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of humans.
Specimen/Volumes	Refer To Manual
Standard Range	Refer To Manual

Description

In this assay the FIBRINOGEN present in samples reacts with the anti-FIBRINOGEN antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-FIB antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound FIB. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of FIB in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of FIB in the test sample. The quantity of FIB in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Intended Use

The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of humans.

Specimen/Volumes

Refer To Manual

Standard Range

Refer To Manual

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