Stemolecule Tranylcypromine hydrochloride is a cell-permeable small molecule that inhibits lysine-specific demethylase 1 (LSD1) in vivo. LSD1 is a histone demethylase capable of demethylating lysine residues1. When combined with CHIR99021, an inhibitor of glycogen synthase kinase 3 (GSK3), tranylcypromine is able to generate human-induced pluripotent stem cells in the absence of the transcription factor Sox22. Tranylcypromine hydrochloride, therefore, can have applications to replace transcription factors in both mouse and human cell reprogramming.
Product Specifications10 mg
Alternate Name(1R,2S)-2-phenylcyclopropanamine hydrochloride
Chemical FormulaC9H11N·HCl
Molecular Weight169.65
CAS Number1986-47-6
PurityGreater than 97% by TLC analysis
FormulationWhite to off-white powder
SolubilityFor a 10 mM concentrated stock solution of Tranylcypromine hydrochloride, add 2.95 ml of DMSO to 5 mg of the compound. If a precipitate is observed, warm the solution to 37°C for 2 to 5 minutes. For cell culture, the media should be prewarmed prior to adding the reconstituted compound. Note: for most cells, the maximum tolerance to DMSO is less than 0.5%. This molecule is soluble in DMSO at 100 mM, water at 29 mM, and ethanol at 59 mM.
Storage and StabilityStore powder at 4°C protected from light. Following reconstitution, store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.
Quality ControlThe purity of Tranylcypromine hydrochloride was determined by TLC analysis. The accurate mass was determined by mass spectrometry. Cellular toxicity of Tranylcypromine hydrochloride was tested on mouse embryonic stem cells.
| Supplier | Product Name | Cat # | Applications |
| Stemgent | A83-01 | 04-0014 | TGF-β1活化素受体样激酶（ALKs）YZ剂，选择性YZALK-4, -5 and -7；YZTGF-β诱导的上皮细胞向间充质细胞转化（EAT）。 |
| Stemgent | ALK5 Inhibitor | 04-0015 | TGF-β1家族I型活化素受体样激酶（ALK5）选择性YZ剂，YZALK5自磷酸化；在重编程过程中发挥作用； |
| Stemgent | AM580 | 04-0077 | 高度特异性视黄酸受体α（RARα）激动剂；在重编程早期，使用CD437处理MEF，能够提高重编程效率高达100倍； |
| Stemgent | BIO | 04-0003 | Wnt信号通路中GSK-3α/βYZ剂；BIO介导的Wnt信号级联通路在无饲养层条件下维持人、小鼠ES细胞自我更新特性； |
| Stemgent | BIX01294 | 04-0002 | 组蛋白赖氨酸甲基转移酶（HMTase）YZ剂；在iPS生成中发挥作用； |
| Stemgent | CD437 | 04-0078 | 视黄酸受体γ（RARγ）选择性激动剂；YZ肿瘤生长，诱导凋亡；在重编程早期，使用CD437处理MEF，能够提高重编程效率高达20倍； |
| Stemgent | CHIR99021 | 04-0004 | 强选择性糖原合成激酶3β(GSK-3β)YZ剂，维持ESC自我更新特性； |
| Stemgent | Doxycycline hyclate | 04-0016 | 光谱四环素衍生抗生素；基质金属蛋白酶（MMPs）YZ剂；在四环素调控的转录激活重编程中，诱导体细胞重编程为iPS细胞。 |
| Stemgent | GSK429286A | 04-0049 | Rho相关激酶（ROCK）选择性YZ剂，YZ细胞凋亡、提高人ES细胞存活率及成克隆率； |
| Stemgent | PD0325901 | 04-0006 | 选择性结合并YZMAPK/ERK激酶（MEK）活性，YZ肿瘤细胞增殖；与SB431542配合，提高原代成纤维细胞重编程为iPS细胞的效率； |
| Stemgent | PD173074 | 04-0008 | 成纤维细胞生长因子YZ剂，维持小鼠、人干细胞自我更新特性 |
| Stemgent | Pifithrin-μ | 04-0039 | YZP53线粒体通路；提高重编程效率； |
| Stemgent | R(+)Bay K 8644 | 04-0013 | L型钙离子通道激动剂；提高重编程效率，与BIX01294联合使用，在只存在Oct4 和Klf4两个转录因子的情况下，能够重编程MEF成iPS细胞； |
| Stemgent | RG108 | 04-0001 | DNA转甲基酶YZ剂；与BIX01294联合，促进MEF重编程为iPS细胞；维持ES细胞未分化状态；在人、小鼠重编程过程中替代某些转录因子； |
| Stemgent | ROCK II Inhibitor | 04-0030 | II型Rho相关激酶（ROCK）特异性YZ剂，YZ细胞凋亡、提高ES细胞存活率及成克隆率； |
| Stemgent | SB431542 | 04-0010 | TGF-β1活化素受体样激酶（ALKs）YZ剂，选择性YZALK-4, -5 and -7；促进ES细胞来源的内皮细胞增殖、分化； |
| Stemgent | SC1 (Pluripotin) | 04-0011 | 长时间维持小鼠胚胎干细胞的自我更新 |
| Stemgent | Sodium Butyrate | 04-0005 | 抗肿瘤；与Activin A、aFGF配合使用，引导小鼠ES细胞向肝细胞分化；提高转染效率； |
| Stemgent | PS48 | 04-0076 | 激活磷酸肌醇依赖的蛋白激酶1（PDK1）；在重编程过程中，促进细胞代谢由线粒体氧化向糖酵解转化；提高重编程效率15倍以上； |
| Stemgent | Thiazovivin | 04-0017 | 直接作用于 Rho相关激酶（ROCK）；促进细胞存活；Thiazovivin与TGF-β受体/MEK通路的YZ剂联合使用,提高重编程效率200倍以上； |
| Stemgent | Tranylcypromine hydrochloride | 04-0033 | 与CHIR9902配合，在无转录因子Sox2情况下，重编程体细胞为iPS细胞； |
| Stemgent | Valproic Acid (VPA) | 04-0007 | 组蛋白去乙酰化酶（HDAC）YZ剂，提高重编程率至少100倍；VPA与细胞因子联合，诱导小鼠ES细胞分化为肝前体细胞，进而分化为肝细胞； |
| Stemgent | Y27632 | 04-0012 | Rho相关激酶（ROCK） YZ剂，YZ细胞凋亡、提高ES细胞存活率及成克隆率； |
