Stage-specific embryonic antigen-1 (SSEA-1), also known as CD15, is an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma (EC) cells, murine embryonic stem (ES) cells, and murine and human germ cells. SSEA-1 is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration, and differentiation. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.
Product Specifications100 μl
Concentration0.5 mg/ml
CloneMC-480
IsotypeMouse IgM, κ
ImmunogenMouse F9 teratocarcinoma stem cells (X-irradiated)
ReactivityMouse, Human
PreparationThis antibody was purified by affinity chromatography and conjugated with DyLight 550 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. The formulation is 0.2 μm filter sterilized and free from bacteria, fungi, mycoplasma, and virus.
FormulationPhosphate-buffered solution, pH 7.2, containing no preservative
Storage and StabilityStore at 4°C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed.
Quality ControlTested by immunocytochemistry to ensure product quality.
Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay.
The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. For an application specific protocol, please reference Protocol: Immunocytochemistry of Live Cells online at www.stemgent.com/support/protocols.
| 品Pai | 货号 | 产品名称 | |
| Stemgent | Reprogramming | 00-0067 | Stemgent® mRNA Reprogramming Factors Set: hOKSML (1 Set) |
| Stemgent | Reprogramming | 00-0068 | Stemgent® VSVg Retrovirus Reprogramming Set: Human OKSM (1 set) |
| Stemgent | Reprogramming | 00-0070 | Pluriton™ Reprogramming Medium (500 mL) |
| Stemgent | Reprogramming | 00-0071 | Stemgent® mRNA Reprogramming Kit (1 kit) |
| Stemgent | Reprogramming | 00-0073 | Stemgent®microRNA Booster Kit (1 kit) |
| Stemgent | Reprogramming | 05-0014 | Stemgent® Oct4 mRNA, Human (20 μg) |
| Stemgent | Reprogramming | 05-0015 | Stemgent® Klf4 mRNA, Human (20 μg) |
| Stemgent | Reprogramming | 05-0016 | Stemgent® Sox2 mRNA, Human (20 μg) |
| Stemgent | Reprogramming | 05-0017 | Stemgent® Lin28 mRNA, Human (20 μg) |
| Stemgent | Reprogramming | 05-0018 | Stemgent® c-Myc mRNA, Human (20 μg) |
| Stemgent | Reprogramming | 05-0019 | Stemgent® nGFP mRNA (20 μg) |
| Stemgent | Reprogramming | 05-0021 | Stemgent® Nanog mRNA, Human (20 μg) |
| Stemgent | Reprogramming | 05-0022 | Stemgent® L-Myc mRNA, Human (20 μg) |
| Stemgent | Differentiation | 05-0029 | Stemgent® MyoD mRNA, Human (20 µg) |
| Stemgent | Differentiation | 05-0031 | Stemgent® Myf5 mRNA, Human (20 µg) |
| Stemgent | Differentiation | 05-0032 | Stemgent® Myog mRNA, Human (20 µg) |
| Stemgent | Differentiation | 05-0033 | Stemgent® Gata4 mRNA, Human (20 µg) |
| Stemgent | Differentiation | 05-0034 | Stemgent® Mef2c mRNA, Human (20 µg) |
| Stemgent | Differentiation | 05-0035 | Stemgent® Tbx5 mRNA, Human (20 µg) |
| Stemgent | Transfection | 00-0069 | Stemfect™ RNA Transfection Kit (1 Kit) |
| Stemgent | Transfection | 05-0020 | Stemgent® eGFP mRNA (20 μg) |
| Stemgent | Antibodies | 00-0055 | Stemgent® Alkaline Phosphatase Staining Kit II (50 assays) |
| Stemgent | Antibodies | 09-0001 | Stemgent® SSEA-1 Antibody (PE), Mouse anti-Mouse/Human (1 mL) |
| Stemgent | Antibodies | 09-0003 | Stemgent® SSEA-4 Antibody (PE), Mouse anti-Human (1 mL) |
| Stemgent | Antibodies | 09-0005 | Stemgent® SSEA-1 Antibody (Purified), Mouse anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0006 | Stemgent® SSEA-4 Antibody (Purified), Mouse anti-Human (100 μL) |
| Stemgent | Antibodies | 09-0009 | Stemgent® TRA-1-60 Antibody (PE), Mouse anti-Human (1 mL) |
| Stemgent | Antibodies | 09-0010 | Stemgent® TRA-1-60 Antibody (Purified), Mouse anti-Human (100 μL) |
| Stemgent | Antibodies | 09-0011 | Stemgent® TRA-1-81 Antibody (Purified), Mouse anti-Human (100 μL) |
| Stemgent | Antibodies | 00-0009 | Stemgent® Alkaline Phosphatase Staining Kit (50 assays) |
| Stemgent | Antibodies | 09-0014 | Stemgent® SSEA-3 Antibody (Purified), Rat anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0020 | Stemgent® Nanog Antibody (Affinity Purified), Rabbit anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0021 | Stemgent® Klf4 Antibody (Purified), Mouse anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0023 | Stemgent® Oct4 Antibody (Affinity Purified), Rabbit anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0024 | Stemgent® Sox2 Antibody (Affinity Purified), Rabbit anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0034 | Stemgent® Goat anti-Rabbit IgG Antibody (DyLight™ 488) (200 μL) |
| Stemgent | Antibodies | 09-0044 | Stemgent® SSEA-3 Antibody (PE), Rat anti-Human (1 mL) |
| Stemgent | Antibodies | 09-0067 | StainAlive™ SSEA-1 Antibody (DyLight™ 488), Mouse anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0068 | StainAlive™ TRA-1-60 Antibody (DyLight™ 488), Mouse anti-Human (100 μL) |
| Stemgent | Antibodies | 09-0069 | StainAlive™ TRA-1-81 Antibody (DyLight™ 488), Mouse anti-Human (100 μL) |
| Stemgent | Antibodies | 09-0072 | StainAlive™ Mouse IgM, κ Isotype Control (DyLight™ 488) (100 μL) |
| Stemgent | Antibodies | 09-0075 | Stemgent® Pax6 Antibody (Purified), Rabbit anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0095 | StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human (100 μL) |
| Stemgent | Antibodies | 09-0097 | StainAlive™ SSEA-4 Antibody (DyLight™ 550), Mouse anti-Human (100 μL) |
| Stemgent | Cytokines | 03-0001 | Stemfactor™ Activin A, Human Recombinant (5 μg) |
| Stemgent | Cytokines | 03-0004 | Stemfactor™ TGF-β1, Human Recombinant (5 μg) |
| Stemgent | Cytokines | 03-0006 | Stemfactor™ Noggin, Human Recombinant (10 μg) |
| Stemgent | Cytokines | 03-0007 | Stemfactor™ BMP-4, Human Recombinant (10 μg) |
| Stemgent | Cytokines | 03-0008 | Stemfactor™ Sonic Hedgehog, Human Recombinant (25 μg) |
| Stemgent | Cytokines | 03-0011 | Stemfactor™ LIF, Mouse Recombinant (1 mL, 10 μg/mL) |
| Stemgent | Cytokines | 03-0011-100 | Stemfactor™ LIF, Mouse Recombinant (1 mL, 100 μg/mL) |
| Stemgent | Cytokines | 03-0016 | Stemfactor™ LIF, Human Recombinant (1 mL, 10 μg/mL) |
| Stemgent | Cytokines | 03-0016-100 | Stemfactor™ LIF, Human Recombinant (1 mL, 100 μg/mL) |
| Stemgent | Media | 00-0047-500 | WIT-T™ Culture Medium (500 mL) |
| Stemgent | Media | 00-0051-500 | WIT-P-NC™ Culture Medium (500 mL) |
| Stemgent | Media | 00-0052-500 | WIT-I-NC™ Culture Medium (500 mL) |
| Stemgent | Media | 00-0072 | Stemgent® MesoFate™ Differentiation Media (500 mL) |
| Stemgent | Media | 00-0074 | Renaissance™Essential Tumor Medium (500 mL) |
| Stemgent | Media | 01-0005 | NutriStem® XF/FF Culture Medium (500 mL) |
| Stemgent | Media | 01-0005-100 | NutriStem® XF/FF Culture Medium (100 mL) |
| Stemgent | Media | 01-0013-50 | CryoStem™ Freezing Medium (50 mL) |
| Stemgent | Media | 01-0014-500 | Stemgent® hES Cell Cloning & Recovery Supplement (5 x 100 μL) |
