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Stemgent® TRA-1-81 Antibody (Purified), Mouse anti-Human (100 μL)

产品信息
  • Product Overview

    The TRA-1-81 monoclonal antibody reacts with a pluripotent-stem-cell-specific antigen expressed upon the surface of human embryonic stem (ES) cells, embyronal carcinoma (EC) cells, and embryonic germ (EG) cells. The expression of TRA-1-81 antigen is stage-specific; as human EC and ES cells undergo differentiation, expression of TRA-1-81 antigen is lost, making it a widely used marker to characterize human ES cells and to monitor their differentiation. TRA-1-81 antibody recognizes a carbohydrate epitope expressed on podocalyxin, a sialoglycoprotein structurally related to CD34. Podocalyxin is a transmembrane glycoprotein which has been implicated in the development of aggressiveness in a variety of cancers, including breast and prostate cancer

    Product Specifications
    Size

    100 μl

    Concentration

    0.5 mg/ml

    Clone

    Tra-1-81

    Isotype

    Mouse IgM, κ

    Immunogen

    Human embryonal carcinoma cell line 2102Ep

    Reactivity

    Human

    Preparation

    This antibody was purified by affinity chromatography.

    Formulation

    Phosphate-buffered solution, pH 7.2, and 0.09% sodium azide

    Storage and Stability

    Store at 4˚C protected from light. Do not freeze. Stable for 6 months from date of receipt when stored as directed.

    Quality Control

    Tested by immunocytochemistry and flow cytometry to ensure product quality.

    Recommended Usage

    The suggested use of this antibody is a 1:100 dilution for immunocytochemistry and 0.25 µg per 1 x 106 viable cells in 100 µl for flow cytometry. For application specific protocols, please referenceProtocol: Immunocytochemistry and Protocol: Flow Cytometry online at www.stemgent.com/support/protocols.

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