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PerCP anti-human CD42b

产品信息
  • 上海科敏生物经销国内外知名品Pai试剂抗体,折扣低,货期快,欢迎新老客户咨询订购!
    PerCP anti-human CD42b参数介绍>>>>
    CloneHIP1
    IsotypeMouse IgG1, κ
    ReactivityHuman, Cross-Reactivity: Chimpanzee
    FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
    PreparationThe antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
    Recommended UsageEach lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

    * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
    Applications

    FC - Quality tested

    Application Notes

    Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation2. The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303908).

    Application References

    1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
    2. Takahashi R, et al. 1999. Blood 93:1951.

    Description

    CD42b is a 145 kD glycoprotein known as gpIbα. It is composed in a heterodimer with a β chain (CD42c, gpIbβ) through a disulfide bond. CD42b is expressed on platelets and megakaryocytes. CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for Von Willibrand factor and thrombin.

    StorageThe antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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