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PerCP anti-mouse CD45.1

产品信息
  • 上海科敏生物经销国内外知名品Pai试剂抗体，折扣低，货期快，欢迎新老客户咨询订购！
    PerCP anti-mouse CD45.1参数介绍>>>>
    CloneA20
    IsotypeMouse (A.SW) IgG2a, κ
    ReactivityMouse
    ImmunogenSJL mouse thymocytes and splenocytes
    FormulationPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
    PreparationThe antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
    Concentration0.2mg/ml
    Recommended Usage

    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

    * PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

    Applications

    FC - Quality tested

    Application Notes

    The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)), and immunofluorescence microscopy9.

    Application References

    1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
    2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
    3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
    4. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
    5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC)
    6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC)
    7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC)
    8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
    9. Mende I, et al. 2006. Blood 107:1383. (IF, IHC, FC)
    10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
    11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
    12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
    13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
    14. Takada K, et al. 2009. J. Exp. Med. 206:2253. PubMed
    15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
    16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
    17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
    18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
    19. Ninjik A, et al. 2012. J Immunol. 189:102. PubMed.

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    Description

    CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

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    StorageThe antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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