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APC anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)

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    APC anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)参数介绍>>>>
    CloneC11.5
    IsotypeMouse IgG1, κ
    ReactivityHuman, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
    ImmunogenCHO-expressed, recombinant human IL-12 p70 heterod
    Formulationµg format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
    Test format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (USA origin).
    PreparationThe antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
    Recommended Usage

    Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more at www.biolegend.com/testsize regarding the test size change.

    "
    Applications

    ICFC - Quality tested

    Application Notes

    Flow Cytometry3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
    Neutralization6: The LEAF™ purified antibody (Endotoxin Additional reported applications (for the relevant formats) include: Western blotting,ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4 of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry.
    Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.

    Application References

    1. D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
    2. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
    3. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
    4. Leifeld L, et al. 2002. Hepatology 36:1001.
    5. Pekkari K, et al. 2001. Blood 97:3184.
    6. Bolovan-Fritts CA, et al. 2004. J. Virol. 78:13173.

    Description

    The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.

    StorageThe antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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