| Recommended Usage | It is recommended to add 5 µl of Human TruStain FcX™ per 100 µl of cell suspension, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps. |
| Applications | FC - Quality tested |
| Application Notes | This buffer contains specialized human IgG. It is not recommended to be used for staining human IgG. Handle as biohazard agent. |
| Application References | 1. de la Cruz J, et al. 2011. J. Immunol. 186:2282. PubMed |
| Description | Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells. |
| Storage | Store at 4°C. |
