| Clone | UCHT1, HCD14, 3G8, HIB19, 2H7, HCD56 |
| Reactivity | Human |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA). |
| Preparation | The antibodies are purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody. |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | FC - Quality tested |
| Application References | 1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC) |
| Description | This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells. |
| Storage | The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
