Biotin anti-mouse/human CD44
- 品牌：Biolegend
- 型号：103003
- 产地：美国
- 供应商：上海恒斐生物科技有限公司
- 供应商报价：￥715
- 标签：Biotin anti-mouse/human CD44、Biotin anti-mouse/human CD44价格、Biotin anti-mouse/human CD44厂家、、、上海恒斐生物科技有限公司
产品库
| Clone | IM7 |
| Isotype | Rat IgG2b, κ |
| Reactivity | Mouse, Human, Cross-Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Squirrel Monkey, Horse (Equine), Cattle (Bovine, Cow), Swine (Pig, Porcine), Dog (Canine), Cat (Feline) |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. |
| Concentration | 0.5mg/ml |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Applications | FC - Quality tested |
| Application Notes | Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, and in vivo inhibition of DTH4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 103046) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). |
| Application References | 1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD) |
| Description | CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. |
| Storage | The antibody solution should be stored undiluted at 4°C. Do not freeze. |
