核糖核酸酶YZ剂，人，重组 | RIBONUCLEASE INHIBITOR (HUMAN，RECOMBINANT)

本产品6折促销，活动期间以下价格不执行

Cat No

CAS No.

NAME/ Chinese Name

Storage

Molecular Formular

MW

Purity

Package/Your Price

C1506A

120178-77-0

RIBONUCLEASE INHIBITOR (HUMAN，RECOMBINANT) | 核糖核酸酶YZ剂，人，重组

−20°C

49 kDa

40U/ul，95% (SDS-PAGE)

2.5KU

1,207

10KU

2,752

C1506B

RIBONUCLEASE INHIBITOR (HUMAN，RECOMBINANT) | 核糖核酸酶YZ剂，人，重组

40U/ul，≥90%（SDS-PAGE)

2.5KU

1,987

10KU

4,163

RIBONUCLEASE INHIBITOR (RI) is a large (~450 residues, ~49 kDa), acidic (pI ~4.7), leucine-rich repeat protein that forms extremely tight complexes with certain ribonucleases. It is a major cellular protein, comprising ~0.1% of all cellular protein by weight, and appears to play an important role in regulating the lifetime of RNA. Both RIs form human placenta and mouse are useful and extensively used for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.
MURINE RNASE INHIBITOR
RNase Inhibitor, Murine is a 50 kDa recombinant protein of murine origin. The inhibitor specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
Recombinant murine RNase inhibitor does not contain the pair of cysteines identified in the human version that is very sensitive to oxidation, which causes inactivation of the inhibitor (1). As a result, RNase Inhibitor, Murine has significantly improved resistance to oxidation compared to the human/porcine RNase inhibitors, and is stable at low DTT concentrations (less than 1 mM). This makes it ideal for reactions where high concentration DTT is adverse to the reaction (eg. Real-time RT-PCR).
Highlights
•Improved resistance to oxidation, compared to human/porcine Rnase inhibitor
•Ideal for reactions where low DTT concentrations are required (e.g. Real-time PCR)
HUMAN RNASE INHIBITOR RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).
The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014 (2).
Highlights
•Isolated from a recombinant source
•Inhibits common eukaryotic RNases
•Compatible with Taq Polymerase, AMV or M-MuLV Reverse Transcriptases
•Active over a broad pH range (pH 5-8)