运输方式： Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
物种来源： Rat
品系： Rat Primary Cells
细胞类型： Endothelial Cells
供应商： Creative Bioarray
规格： 5ML
Rat Spleen Endothelial Cells from Creative Bioarray are isolated from spleen tissue of 6-8 week old laboratory Sprague-Dawley rat. Rat Spleen Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen. The method we use to isolate endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 (CD31) antibody, following the application of magnetic beads pre-coated with secondary antibody. Product Link: http://www.creative-bioarray.com/Rat-Spleen-Endothelial-Cells-CSC-C4133X-item-2410.htm
