CRL-1444 A7r5 大鼠胸大动脉平滑肌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范，提供的细胞株背景清楚，提供参考文献和Z优培养条件!
CRL-1444 A7r5 大鼠胸大动脉平滑肌细胞
ATCC® Number:
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CRL-1444™
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Price:
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$275.00
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Designations:
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A7r5
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Depositors:
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W Carlisle
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Biosafety Level:
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1
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Shipped:
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frozen
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Medium & Serum:
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See Propagation
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Growth Properties:
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adherent
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Organism:
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Rattus norvegicus (rat)
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Morphology:
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fibroblast
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Source:
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Organ: aorta, thoracic
Strain: DB1X
Tissue: smooth muscle
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Cellular Products:
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myokinase; creatine phosphokinase (muscle isoenzyme); myosin
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Permits/Forms:
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Applications:
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transfection host (Roche FuGENE® Transfection Reagents
technology from amaxa)
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Age:
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embryo
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Comments:
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The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase.
Muscle type CPK is synthesized after cell division has ceased.
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Propagation:
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Subculturing:
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Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
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Preservation:
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Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
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Related Products:
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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References:
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1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301
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