Mouse monoclonal [Glu-Glu] to Glu-Glu tag - Affinity Matrix

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抗体参数如下>>>>

产品名称：ab24587/Anti-Glu-Glu tag antibody
产品描述：Mouse monoclonal [Glu-Glu] to Glu-Glu tag - Affinity Matrix
货号：ab24587
品Pai：abcam
抗体别名： EYMPME epitope tag antibody；EYMPME epitope tag antibody；EYMPME tag antibody； EYMPME tag antibody；Glu-Glu epitope tag antibody；Glu-Glu epitope tag antibody；
克隆类型：Monoclonal
纯化方法：Protein G purified
纯化测试条件：This antibody was purified using protein-G chromatography and was subsequently immobilized onto a Sepharose(TM) Fast Flow matrix.
交叉反应：
测试应用：IP
抗体亚型：IgG
特异性：This affinity Matrix can be used for immunopurification of Glu-Glu tagged fusion proteins from crude starting material.Binding: Tagged protein will bind to matrix in common physiologic buffers with pH in the range of 6.0-7.5, salt from 50-500 mM and in the presence of reasonable levels of detergent. Excess reducing agent should be avoided as the disulfide bridges holding antibody heavy and light chains may be compromised.Washing: After binding, washes with several bead volumes of buffer are recommended.Elution: Several options are available for elution.1. SDS gel loading buffer may be applied directly to the beads in order to display all bound protein on a polyacrylamide gel/western. Note that gel loading buffer containing reducing agent will also release some antibody heavy and light chains (approx 25 and 50kD, respectively).2. Competitive elution with epitope peptide. For epitope tag affinity matrices, prepare an elution buffer with epitope tag peptide at 400 ug/ml in 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM EDTA (pH 8.0).3. Chemical elution. Elution by pH or chaotropic salts is also possible. For elution by pH, either 0.1 M glycine pH 2.8 or 40 mM diethyl-amine pH 11.0 may be used.
性状：Liquid
保存液：The slurry contains,Preservative: 0.03% Thimerosal (merthiolate)Constituents: PBS
保存条件：Store at +4°C.