G1120 HE染色试剂盒 （苏木素-伊红）染色试剂盒

HE染色试剂盒 （苏木素-伊红）染色试剂盒


HE染色试剂盒产品说明书

HE（苏木素-伊红）染色试剂盒使用说明书货号：G1120
规格：10ml/100ml
保存：本试剂盒常温保存，复检期至少1年。

注意：环境温度低时，分化液可能会结晶析出，将分化液37℃水浴融化后即可，不影响使用。
 

HE染色试剂盒 （苏木素-伊红）染色试剂盒产品说明： 
        苏mu精-伊红染色法 ( Hematoxylin-Eosin staining )，简称HE染色法，切片技术里常用的染色法之一。苏mu精染液为碱性，主要使细胞核内的染色质与胞质内的核糖体着紫蓝色；伊红为酸性染料，主要使细胞质和细胞外基质中的成分着红色。 染色过程需要优化，着色情况与组织或细胞的种类有关，也随其生活周期及病理变化而改变。例如，很多细胞在新生时期胞浆对伊红着色较淡或轻度嗜碱，当其衰老时或发生退行性变则呈现嗜伊红浓染。胶原纤维在老化和出现透明变性时，伊红着色由浅变深。本产品所包含试剂均为工作液，可直接使用。

操作说明： 石蜡组织切片的HE染色（以下步骤仅供参考）。
1．取材组织块，经固定后，常规石蜡包埋，切片。
2．切片用二甲苯脱蜡，经各级乙醇至水洗：二甲苯（I）5min→二甲苯（Ⅱ）5min→100％乙醇2min→95％乙醇1min→80％乙醇1min→75％乙醇1min→蒸馏水洗2min。
3．苏木素染液染色5-20min(可以根据染色结果和要求调整时间)，自来水冲洗。
4．分化液分化30s。
5．自来水浸泡15min或温水（约50℃）5min。
6．置伊红染液2min。
7．自来水冲洗。
8．常规脱水，透明，封片：95％乙醇（I）1min→95％乙醇（Ⅱ）1min→100％乙醇（I）1min→100％乙醇（Ⅱ）1min→二甲苯石碳酸（3：1）1min→二甲苯（I）1min→二甲苯（Ⅱ）1min →中性树脂封固，镜下观察。冰冻切片不用脱蜡，可固定后直接染色，其方法与石蜡切片相同。

HE染色试剂盒 （苏木素-伊红）染色试剂盒相关产品：
G1010 姬姆萨染色液
G1040 瑞氏染色液
G1100 伊红染色液
G1140 苏木素染色液（常规染色）
P1120 10×多聚赖氨酸
N8010-5Naphthalene acetic acid(NAA) α－萘乙酸5g
C8190-250Colchicine 秋水仙碱250mg
I8020-13-Indole acetic acid(IAA) 吲哚-3-乙酸1g
I8020-53-Indole acetic acid(IAA) 吲哚-3-乙酸5g
I8030-1Indole-3-butyricacid(IBA) 吲哚-3-丁酸(水溶)1g
I8030-5Indole-3-butyricacid(IBA) 吲哚-4-丁酸(水溶)5g
A8060-2bscisic acid (ABA) 　脱落酸25mg
A8060-100Abscisic acid (ABA) 　脱落酸100mg
F8080-1Forchior fennron 氯吡苯脲 (促进植物细胞分裂)1g
X8040-5Zeatin 玉米素 (细胞分裂素，用于植物组织培养)5mg
T8110-50Trans-Zeatin (反玉米素 植物细胞分裂素)50mg
T8050-100Thidiazuron (TDZ) 噻苯隆100mg
C8790-100Chlormequat 矮壮素100g
P8790-100Paclobutrazol 多效唑100g
D8100-52,4-D 2,4-二氯苯氧乙酸5g
D8100-102,4-D 2,4-二氯苯氧乙酸10g
K8010-250Kinetin 激动素250mg
A8170-16-Benzyl Aminopurine 6-苄氨基喋呤1g

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