产品货号：K-RAFGA
品Pai：Megazyme
产品品名：密三糖或棉子糖/D-半乳糖检测试剂盒
英文品名：Raffinose/D-Galactose Assay Kit
规格型号：120 assays per kit
For the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.
INTRODUCTION:
Grain legumes are an important component of both human and livestock diets. Galactosyl-sucrose oligosaccharides (raffinose, stachyose and verbascose) are major components in many food legumes1, and the antinutritional activity of grain legumes is frequently associated with the presence of these oligosaccharides2. However, recent data now suggest a beneficial role as prebiotics. Raffinose is usually the major galactosyl-sucrose oligosaccharide present in most legume seeds3. It is also a significant component in sugar-beet molasses.
Several solvents can be employed for the extraction of galactosyl-sucrose oligosaccharides from milled legume-seed. These are generally water/alcohol mixtures. Before, or concurrent with, extraction it is essential that endogenous α-galactosidase and invertase are inactivated. This can be achieved by refluxing a sample of the ground material in ethanol or in an aqueous ethanol mixture before the flour is subjected to extraction.
KITS:
Kits suitable for performing 120 determinations are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 8.6) containing sodium azide (0.02% w/v) as a preservative.
Stable for > 2 years at 4°C.
Bottle 2: NAD+.
Stable for
> 5 years at -20°C.
Bottle 3: D-Galactose dehydrogenase plus galactose mutarotase suspension (2.5 mL).
Stable for > 2 years at 4°C.
Bottle 4: α-Galactosidase (pH 4.6), lyophilised powder.
Stable for
> 4 years at -20°C.
Bottle 5: Galactose standard solution (5 mL, 0.4 mg/mL) in 0.02% (w/v) sodium azide.
Stable for
> 2 years at 4°C.
Bottle 6: Raffinose control powder (~ 4% w/w raffinose in mannitol; the exact concentration is stated on the vial label).
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