L-谷氨酸 氨快速检测试剂盒 L-Glutamine / Ammonia (Rapid) Assay Kit 产品货号：K-GLNAM

产品货号：K-GLNAM 

品Pai：Megazyme

产品品名：L-谷氨酸 氨快速检测试剂盒

英文品名：L-Glutamine / Ammonia (Rapid) Assay Kit

规格型号：100 assays – 50 of each component

Novel method for the specific, convenient, cost effective and rapid assay of L-glutamine and ammonia in culture media/supernatants and other materials.

INTRODUCTION:

L-Glutamine is an essential component of certain cell culture media. However, the incorporation of this amino acid into growth media presents two major problems; firstly, L-glutamine is labile, and spontaneously breaks down to L-glutamate and free ammonium ions, and secondly, the released ammonium ions are very toxic to the cells. To overcome these issues, either pre-formulated growth media are used strictly within their recommended shelf-lives or L-glutamine is added just before use. In either case, monitoring of L-glutamine and ammonia is frequently performed both prior to, and during culturing.
Individual test kits do exist for L-glutamine and ammonia, but these products can be very slow, owing to either the limiting amounts or nature of the enzymes employed. Megazyme thus developed this kit (K-GLNAM), based on advanced recombinant enzymes, that is both very rapid (~ 15 min) and also measures both analytes in a very simple format. As the kit is based on quantitative endpoint readings, a calibration curve is not required, saving more time and expense. Both manual (see page 5, “A”) and microplate (see page 8, “B”) formats are described. Other rapid cell culture test kits are also available from Megazyme for ammonia (K-AMIAR), L-asparagine / ammonia (K-ASNAM), D-glucose (K-GLUHKR or K-GLUC) and L-lactic acid (K-LATE).
This kit is also suitable for the analysis of L-glutamine in a wide range of other samples, such as dietary supplements and vegetables.

KITS:

Kits suitable for performing 50 assays of both ammonia and
L-glutamine are available from Megazyme. The kits contain the full assay method plus:
Bottle 1: Buffer (11 mL, pH 4.9) plus sodium azide
(0.02% w/v) as
a preservative Stable for > 2 years at 4°C.
Bottle 2: (x2) Buffer (25.5 mL, pH 8.0) plus sodium azide
(0.02% w/v) as
a preservative.
Stable for
> 2 years at 4°C.
Bottle 3: (x2) NADPH. Lyophilised powder.
Stable for > 5 years at -20°C.
Bottle 4: Glutaminase suspension (1.1 mL).
Stable for
> 2 years at 4°C.
Bottle 5: Glutamate dehydrogenase (2.2 mL).
Stable for
> 2 years at 4°C.
Bottle 6: Ammonia standard solution (5 mL, 0.04 mg/mL) in 0.02% (w/v) sodium azide.
Stable for
> 2 years at 4°C.
Bottle 7: L-Glutamine control powder (~ 2 g).
Stable for
> 2 years at 4°C.

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