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L-阿拉伯糖D-半乳糖检测试剂盒 L-Arabinose / D-Galactose Assay Kit 产品货号:K-ARGA

产品信息

  • 产品货号:K-ARGA 

    品Pai:Megazyme

    产品品名:L-阿拉伯糖D-半乳糖检测试剂盒

    英文品名:L-Arabinose / D-Galactose Assay Kit

    规格型号:115 assays (manual) / 1150 assays (microplate) / 1150 assays (auto-analyser) 

    A simple, reliable and accurate UV method for the measurement of L-arabinose and/or D-galactose in various materials including foods, feeds, beverage and plant products. 

    INTRODUCTION:

    Enzymic kits for the determination of L-arabinose and D-galactose
    are very slow. This is due to the low rate of natural chemical
    “mutarotation” between the α- and β-anomeric forms of these
    monosaccharides. Only the β-form is recognised by β-galactose
    dehydrogenase. In incubations containing NAD+, L-arabinose
    or D-galactose and β-galactose dehydrogenase, there is a very
    rapid initial increase in absorbance due to the consumption of β
    -L-arabinose or β-D-galactose, and this is followed by a very slow
    approach to the end-point. This very slow approach results from
    the very low rate of chemical “mutarotation” of the α-anomeric
    conformation of these monosaccharides to the respective β-anomer.
    Using technology developed by Megazyme (patent pending), a
    galactose mutarotase has now been incorporated into the assay
    format to rapidly catalyse this rate-limiting mutarotation step.
    The result is very rapid analysis times of approximately 6 min for
    D-galactose and approximately 12 min for L-arabinose (Figure 1).

    KITS:

    Kits suitable for performing 115 assays in manual format (or 1150 assays
    in microplate or auto-analyser formats) are available from Megazyme.
    The kits contain the full assay method plus:
    Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02% w/v) as
    a preservative. Stable for > 2 years at 4°C.
    Bottle 2: NAD+. Lyophilised powder.
    Stable for > 5 years at -20°C.
    Bottle 3: β-Galactose dehydrogenase plus galactose mutarotase
    suspension, 2.4 mL. Stable for > 2 years at 4°C.
    Bottle 4: L-Arabinose standard solution (5 mL, 0.4 mg/mL in
    0.02% w/v sodium azide).
    Stable for > 2 years at 4°C.
    Bottle 5: D-Galactose standard solution (5 mL, 0.4 mg/mL in
    0.02% w/v sodium azide).
    Stable for > 2 years at 4°C.


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