Anti-HIF-1 Alpha缺氧诱导因子1α 抗体HIF-1α

本公司提供科研HIF-1 Alpha缺氧诱导因子1α 抗体HIF-1α，抗体质量可靠，订购HIF-1 Alpha缺氧诱导因子1α 抗体HIF-1α请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：缺氧诱导因子1α 抗体HIF-1α
英文名称：Anti-HIF-1 Alpha
货号：bs-0737R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 92kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo, dog, pig, cow, chk, Rb
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator(ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.Subunit : Interacts with COPS5 subunit of COP9 signalosome complex, leading to the regulation of its stability. Interacts with TSGA10.Subcellular Location : Cytoplasm. Nucleus.Tissue Specificity : Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.Similarity : Contains 1 basic helix-loop-helix (bHLH) domain.Contains 1 PAC (PAS-associated C-terminal) domain.Contains 2 PAS (PER-ARNT-SIM) domains.缺氧诱导因子1α不仅对于机体在缺氧条件下维持正常的生理功能具有特别重要的意义,并在肿瘤的生长以及神经细胞凋亡等病理过程中起重要作用. HIF1 alpha能调节许多下游基因的表达水平. 哺乳动物细胞在低氧压力条件下出现HIF。HIF是一种转录因子，对细胞的缺氧起稳定作用。产品图片Tissue/cell: rat lung tissue(Smoking); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HIF-1-Alpha Polyclonal Antibody, Unconjugated(bs-0737R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingProtein: line1, mouse brain lysates, 30ug; line2, mouse liver lysates, 30ug; Primary: Anti-HIF-1-Alpha (bs-0737R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 92kDObserved band size : 50kD, 92kDWe are unsure as to the identity of these extra bands