Catalog Number: SG CL-202
1.适用于过氧化物检测的细胞发光增强剂
2.使用方便特别适用于过氧化物
3.增强信号100-600倍
4.只需要非常少的细胞且对细胞无毒性
Diogenes细胞化学发光增强系统是一种过氧化物化学发光增强剂，不会使活细胞变性失活。超氧自由基会在细胞有氧呼吸或者白细胞抵御感染的过程中产生。白细胞在受到fMLP、佛波酯、抗Fc受体的抗体以及LPS刺激时进行氧化反应的程度可以部分作为免疫活力的指标。现在测定白细胞产生的超氧自由基的方法都非常的繁琐而且不直接，例如在Cyanide存在或缺失的条件下测定克拉克氏电极对氧气的吸收，或者测定细胞色素c被还原时发生的光谱改变。Diogene试剂盒利用生成无毒性中间产物的发光反应，非常适用于细胞介导的过氧化物生成检测。过氧化物氧化反应发射化学荧光的强弱与超氧自由基的浓度成正比，而且比使用鲁米诺发出的光强很多。因此Diogenes非常适合于检测细胞的免疫活性，只需要使用化学光度计便可定量光信号的强弱。任何刺激氧化酶产生胞外过氧化物的反应都可以用Diogenes检测。可以在生理条件下或者模仿生理条件下完成过氧化物的检测。
Diogenes Cellular Luminescence Enhancement System is a superoxide chemiluminescent enhancer that is non-denaturing to living cells. Superoxide radical (O2-) is produced intracellularly as a consequence of aerobic metabolism and extracellularly by leukocytes in response to infection. The extent of oxidative burst produced by white blood cells (WBCs) when stimulated by f-met-leu-phe, phorbol esters, anti-Fc receptor antibodies or LPS is a partial indicator of the immunocompetence of the cells tested.
Currently, the production of O2- by leukocytes is monitored by such cumbersome and indirect methods as measuring oxygen uptake in a Clark electrode (both in the presence and absence of cyanide) or measuring spectral changes caused by the reduction of cytochrome c. As a non-cytotoxic intermediate in the mechanism of photon production, Diogenes is ideally suited to the detection of cell-mediated superoxide production. The intensity of light produced by Diogenes in the presence of superoxide is directly proportional to the O2- concentration, but is much higher than that achieved by using luminol. Therefore, Diogenes is ideal for monitoring cellular immunocompetence, utilizing a luminometer to quantify the light output. Any stimulant that activates an oxidase to produce extracellular superoxide is usable with Diogenes. Such means can be physiological ormimetic of the physiologic pathway.
