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CRL-9068 NCI-H929 人瘤细胞株

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    CRL-9068 NCI-H929 人瘤细胞株 的详细介绍
    CRL-9068 NCI-H929 人瘤细胞株
    ATCC® Number: CRL-9068™     Price:  
    Designations: NCI-H929 [H929]
    Depositors:  National Cancer Institute
    Isotype: IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: suspension
    Organism: Homo sapiens (human)
    Morphology: lymphoblast
    Source: Organ: bone marrow 
    Disease: plasmacytoma; myeloma 
    Cell Type: B lymphocyte;
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
     
    Receptors: transferrin
    Antigen Expression: PCA-1; CD38 +
    DNA Profile (STR): Amelogenin: X 
    CSF1PO: 11 
    D13S317: 12 
    D16S539: 9,13 
    D5S818: 11,12 
    D7S820: 10,12 
    THO1: 9.3 
    TPOX: 8,11 
    vWA: 14,15
    Cytogenetic Analysis: Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality.
    Age: 62 years
    Gender: female
    Ethnicity: Caucasian
    Comments: This cell line was established from a malignant effusion in a patient with myeloma. 
    The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development. 
    They are negative for Epstein-Barr virus nuclear antigen (EBNA). 
    The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA. 
    There is also an activated ras allele.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C
    Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 10(5) viable cells/ml. 
    Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml. 
    Medium Renewal: 2 to 3 times per week
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
    Storage temperature: liquid nitrogen vapor phase
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
    recommended serum:ATCC 30-2020
    References: 22372: Hollis GF, et al. Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed: 3275865
    23349: Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157
    23351: Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435
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