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Corning® Epoxide Slide Starter Kit (Product #40040) 环氧化物包被微阵列芯片

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  • Corning® Epoxide Slide Starter Kit (Product #40040) 环氧化物包被微阵列芯片


    环氧化物包被微阵列芯片
    稳定的表面可共价结合短寡聚核苷酸(有氨基修饰或者无氨基修饰,~30-mer)、长寡聚核苷酸(~>50-mer)和cDNA。配合Pronto!TM 通用杂交试剂盒可使芯片整体性能达到。
    特点
    多功能性: 对短寡聚核苷酸、长寡聚核苷酸和cDNA具有同样效果。可印迹有氨基修饰或无氨基修饰的寡聚核苷酸。DNA耦合无需UV交联或烘烤步骤。
    重复性:玻片间差异极小,不到3%CV
    灵敏度:在4μg总RNA中可检测1pg RNA
    特异性:可区分同源性寡聚核苷酸90%(30-mer寡聚核苷酸中有3个错配) 


    Corning® Epoxide Coated Slides are designed for covalent attachment of oligonucleotides (both short and long oligos, with and without amino-modification).
     

    The Corning® Epoxide Slide Starter Kit offers 10 Epoxide Coated Slides, 0.8 mL of Pronto!™ Long-Oligo/cDNA Hybridization Solution, and 0.8 mL Pronto!™ Short Oligo Hybridization Solution. This kit is being offered as a way of providing a low cost trial of critical components for the optimization of oligo-array production and the subsequent hybridization to labeled cDNA.

    The Corning® Epoxide Slides work best when oligos are dissolved in 150 mM sodium phosphate, ph 8.5 to 9.0. A small amount of detergent may be added to increase spot size, depending on printing conditions and the type of pins used.

    When Corning® Epoxide Coated Slides are used in conjunction with the Pronto!™ Universal Hybridization Kit, the following features are evident:
    • Sensitivity to 1 pg target RNA in a 4 µg total RNA direct labeling reaction
    • Interslide CVs below 10%
    • Detection of low expression targets above extremely low backgrounds
     


    product details at-a-glance
    Material: Glass
    Qty./Pk.: 1
    Qty./CS.: 1
    Barcoded: Yes
    Dimensions (LxWxH): 25.3x75.5x1.1 mm (approx)
    Sterile: No
    Coating: Epoxide
    product details

    10 Epoxide Coated Slides, 0.8mL Short Oligo and 0.8mL cDNA/Long Oligo Hybridization Solution

    Corning® Epoxide Slide Starter Kit (Product #40040) 环氧化物包被微阵列芯片
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