TMB High Sensitivity Substrate Solution

The TMB High Sensitivity (TMB HS) Substrate solution is an one-step working solution for HRP (horse-radish peroxidase) labeled conjugates. TMB HS substrate solution provides high signal and high sensitivity for ELISA. It is recommended for cytokine ELISA reagent sets. The substrate can be catalyzed with peroxidase to produce a pale blue color which can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2N H₂SO₄ or 1M H₃PO₄ (resulting in a yellow color), and read at 450 nm.

421501	TMB High Sensitivity Substrate Solution	60 ml
421601	ELISA Wash Buffer (20X)	500 ml
421701	ELISA Coating Buffer (5X)	30 ml
422101	FluoroFix Buffer	200 tests
422201	Annexin V Binding Buffer	100 ml
422301	Human TruStain FcX (Fc Receptor Blocking Solution)	50 tests
422401	RBC Lysis/Fixation Solution (10X)	50 ml
430101	Human IFN-γ ELISA MAX Standard	5 Plates
409209	DyLight 405 Goat anti-mouse IgG2a	100 ug
409301	Purified anti-human IgG Fc	50 ug
409303	PE anti-human IgG Fc	25 tests
420201	Cell Staining Buffer	500 ml
420301	RBC Lysis Buffer (10X)	100 ml
420404	7-AAD Viability Staining Solution	500 tests
420601	Brefeldin A Solution (1,000X)	1 ml
420701	Monensin Solution (1,000X)	1 ml
420801	Fixation Buffer	100 ml
421002	Permeabilization Wash Buffer (10X)	100 ml
421101	TMB Substrate Reagent Set	100 ml×2
421203	ELISA Assay Diluent (5X)	100 ml
408905	FITC anti-rat IgM	100 ug