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阻断剂(常备货),Blocking Reagent

产品信息
  • 德国罗氏试剂,部分常用现货,订货两到三周,每周三订单
    http://www.runwelltac.comBlocking ReagentFor nucleic acid hybridization and detection产品描述

    Preparation Note

    Working concentration: Dilution of Antibody 1:5,000
    Working solution: To prepare a 10x blocking stock solution Blocking Reagent is dissolved in maleic acid buffer to a final concentration of 10% (w/v) with shaking and heating either on a heating block or in a microwave oven.
    Storage conditions (working solution): 10x stock solution of Blocking Reagent is autoclaved and stored at 2 to 8 °C. Alternativly, aliquots can be stored at -15 to -25 °C.

    Application

    The Blocking Reagent is used to decrease the background in nonradioactive filter hybridization and the detection of nucleic acid hybrids.It has also been used as a blocking agent in immunohistochemistry.


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