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Caspase-10 (Pro) Antibody 单克隆抗原抗体 1035-1

产品信息

  • Description

    Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-10 (MCH4, FLICE2) is one of the initiator caspases (1,2), and is localized to various tissues. Pro-caspase-10 has two death effector domains (DEDs) that bind FADD and recruits both TNFR1 and CD95 to form complexes with these receptors and induce apoptosis (1,2). This death domain complex cleaves pro-caspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 leads to the processing of caspase-3 and caspase-7, initiating a caspase cascade and subsequent apoptosis (3,4). It has been shown that the Caspase-10 protein is not produced in neither mouse nor rat (5).

    Recommended Applications

    WB, IHC, ICC, IP, FC
     
    Applications and Recommended Dilution Factors
     
    WB IHC ICC FC IP
    1:1,000 1:50-1:100 1:100 1:50 1:10

    Species Reactivity*
     
    Human Mouse Rat
    Positive Positive Negative *Cross reactivity determined by western blot only.

     
    Product Data
    A. Western blot analysis of HeLa cell lysate using anti-Pro-Caspase-10 RabMab (cat#1035-1), 1,000 dilution. B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Caspase 10 RabMab (cat#1035-1).
    C. Caspase 10 expression in apoptotic HeLa cells detected by Immunofluorescent staining using anti-Pro-Caspase 10 RAbMab (cat#1035-1). D. Flow cytometric analysis of permeabilized HeLa cells using anti-Pro-Caspase-10 RabMAb (red) (catalog #1035-1)or a rabbit IgG (negative) (green).

    Specificity

    A synthetic peptide corresponding to N-terminal residues of human Caspase-10 subunit p23/17 was used as immunogen. The antibody does not cross-react with other Caspase family members.

    Storage Buffer & Conditions

    50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

    Alternative Names

    CASP10, MCH4, Caspase-10 subunit p12, ICE-like apoptotic protease 4;Apoptotic protease Mch-4;FAS-associated death domain protein interleukin-1B-converting enzyme 2

    Description References

    1. Vincenz, C. and Dixit, V.M. Fas-associated death domain protein interleukin-1beta-converting enzyme 2 (FLICE2), an ICE/Ced-3 homologue, is proximally involved in CD95- and p55-mediated death signaling. J. Biol. Chem. 272: 6578
    2. Fernandes-Alnemri, T. et al. In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains. Proc. Natl. Acad. Sci., USA 93: 7464
    3. Srinivasula, S.M. et al. Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases. Proc. Natl. Acad. Sci., USA 93: 14486
    4. Wang, J. et al. Inherited human Caspase 10 mutations underlie defective lymphocyte and dendritic cell apoptosis in autoimmune lymphoproliferative syndrome type II. Cell 98: 47
    5. Janicke RU, Science 312(5782): 1874, 2006.

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