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Caspase-7 (Pro) Antibody 兔单克隆抗原抗体 1033-1

产品信息

  • Description

    Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1, Mch3, ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1,2,3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation, Caspase-7 is translocated to the mitochondria, and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2,3) and endoplasmic reticular-specific substrate, sterol regulatory element-binding protein 1 (4).

    Recommended Applications

    WB, IHC, ICC
     
    Applications and Recommended Dilution Factors
     
    WB IHC ICC FC IP
    1:1,000 1:50 1:50    

    Species Reactivity*
     
    Human Mouse Rat
    Positive Negative Negative *Cross reactivity determined by western blot only.

     
    Product Data
    A. Western blot analysis on Jurkat cell lysate using anti-Pro-Caspase-7 RabMAb (cat. #1033-1), 1:1,000 dilution. B. Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using anti- anti-Pro-Caspase-7 RabMAb (cat. #1033-1).

    Specificity

    A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7 was used as immunogen. The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.

    Storage Buffer & Conditions

    50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

    Alternative Names

    CASP7, MCH3, Caspase-7 subunit p11, Apoptotic protease Mch-3;CMH-1

    Description References

    1. Duan, H., et al. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. J. Biol. Chem. 271:
    2. Lippke, J.A., et al. Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32. J. Biol. Chem. 271: 1825
    3. Fernandes-Alnemri, T., et al. Mch3, a novel human apoptotic cysteine protease highly related to CPP32. Cancer Res. 55: 6045
    4. Chandler, J.M., et al. Different Subcellular Distribution of Caspase-3 and Caspase-7 following Fas-induced Apoptosis in Mouse Liver. J. Biol. Chem. 273: 10815
     

     

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