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Caspase-9 (Pro) Antibody 兔单克隆抗原抗体 1023-1

产品信息

  • Description

    Caspase-9 (ICE-LAP6, Mch-6, Apaf-3) is a member of the cysteine aspartic acid protease, or caspase, family. Pro-Caspase-9 exists as a 46 kDa isomer that is cleaved by apoptotic stimulation, and functions as the major initiator caspase in programmed cell death, or apoptosis (1-4). Upon stimulation, cytochrome c is released from mitochondria, and associates with the Pro-Caspase-9/Apaf 1 complex. This complex in turn activates Pro-Caspase-9 by cleaving it into an active 35 kDa fragment and a small 10 kDa fragment (1,2). Activated Caspase-9 cleaves Caspase-3 as well as poly (ADP-ribose) polymerase (PARP) to initiate a caspase cascade and apoptosis (3,4).

    Recommended Applications

    WB, IHC, ICC, FC, IP
     
    Applications and Recommended Dilution Factors
     
    WB IHC ICC FC IP
    1:100-500 1:50-100 1:50 1:100 1:100

    Species Reactivity*
     
    Human Mouse Rat
    Positive Positive Negative *Cross reactivity determined by western blot only.

     
    Product Data
    A. Western blot analysis on HeLa cell lysate using anti-Caspase-9 (pro) RabMAb (catalog #1023-1), dilution 1:500. B. Immunohistochemical staining of paraffin-embedded human cervical carcinoma using anti-Caspase-9 (pro/p35) RabMAb (catalog #1023-1).

    Specificity

    A synthetic peptide corresponding to C-terminus of human Caspase-9 p35 subunit was used as immunogen. The antibody should recognize only pro-form (46kDa) .

    Storage Buffer & Conditions

    50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

    Alternative Names

    CASP9, MCH6, Caspase-9 subunit p10, Apoptotic protease Mch-6;Apoptotic protease-activating factor 3

    Description References

    1. Kuida, K. et al. Reduced Apoptosis and Cytochrome c
    2. Li, P. et al. Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade. Cell 91: 479
    3. Duan, H. et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271: 16720
    4. Srinivasula, S.M. et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 271: 27099
     
     

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