LNCa细胞：人前列腺癌复苏快细胞系 货号：GD-C4720539
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
3'-BroMo-5'-(trifluoroMethyl)acetophenone, 97%
1-Nonanol
3-(METHYLTHIO)PHENYLACETIC ACID
6-BroMoindanone
3-Hydroxy-2-naphthoic Acid 2-Chloroanilide , 98%
(S)-(-)-1-BENZYL-3-ACETAMIDOPYRROLIDINE
2-Phenoxypropionyl Chloride
Dodecyldimethylchlorosilane, 97%
Tri-n-octyl Borate
Octadecyl Vinyl Ether
2-Methyl-3-pentanone
Acid Green 50
TETRABROMOPHTHALIC ACID
Boc-beta-cyclohexyl-D-alanine monohydrate
1,3-Benzothiazole-2-carbaldehyde
3,4-Dichlorophenyl isocyanate
N,N'-Di-n-octyl-3,4,9,10-perylenetetracarboxylic DiiMide
N-Propyl 5-broMopicolinaMide
1,2-DibroMo-3,5-difluorobenzene, 99%
TriMeprazine HeMi-(+)-tartrate Salt, BP
4-Fluorobenzyl Alcohol
quinine sulphate
2,4-Dimethylanisole
Pigment Blue 1
3-phenoxypropane-1,2-diol
1-ACETYL-1-CYCLOHEXENE
2-Amino-3,5-dichloropyridine
SodiuM Metaborate Tetrahydrate
Bis(cyclopentadienyl)tungsten dihydride, 98%
p-Toluenesulfonic anhydride, 97%
Selenious acid
PolyraM
Boc-(R)-α-(4-trifluoroMethylbenzyl)-Pro-OH
N-Methyl 5-broMopyridine-3-sulfonaMide
2-FLUORO-6-(TRIFLUOROMETHYL)BENZOYL CHLORIDE
1L-EPI-2-INOSOSE
Mesobilirubin
Cyclobutyl-4-fluorophenyl ketone, 98%
(S)-(o-(N-Benzylprolyl)amino)(phenyl)-methyleneiminoacetate
2-Bromo-5-fluorophenylacetic acid
BENZAL CHLORIDE
7-AVCA
(S)-(+)-1-Methyl-3-pyrrolidinol
Salicylaldoxime
2-BroMo-1-[5-(2-pyridinyl)-2-thienyl]-1-ethanone
Thiazole-2-carboxylic acid
2,2'-Ethylenedianiline
Z-Abu-oh
1-[3-(4-Chlorophenyl)-5-Methylisoxazol-4-yl]ethan-1-one, 97%
CIS-1 - 4-DICHLORO-2-BUTENE
3,4-Difluoroaniline
Angiotensin
Silver perchlorate
N,N'-trimethyleneurea
3-Nitrobenzyl alcohol
Bis(4-tert-butylcyclohexyl) peroxydicarbonate
2-Methyl-4-(N-MethylsulfaMoyl)phenylboronic acid
Benzalacetone
2-Methyl-4-Methoxycarbonylphenylboronic acid, pinacol ester
6-Methylindole, 99%
4-Propoxybenzonitrile , 97%
Boc-D-Dab(Fmoc)-OH
3-Bromo-2-chloro-4-pyridinamine
3-AMINO-3-(4-ETHYLPHENYL)PROPANOIC ACID
HEXAMETHYLACETONE
PENTAMETHYLIODOBENZENE
Trimethylboroxine
Ethyl lactate
1-Heptanethiol
2-Methyl-6-ethoxyquinoline
4-ForMylpiperidine
2-Chloro-6-MethylpyriMidine-4,5-diaMine, 95%
4,6-DINITRO-2-METHYLPHENOL
2,6-DiMethylphenoxyacetic Acid
3-Dimethylaminoacrylic acid ethyl ester
Sodium bromite
n-Dodecanophenone
1,1'-Bi-2-naphthol
ACD ELISA Kit
Klotho ELISA Kit
Human EL ELISA Kit
Human DDOST/OST-48 ELISA Kit
CIC ELISA Kit
p16 ELISA Kit
Chicken cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
Human PKCζ ELISA Kit
Human PBP/CXCL7 ELISA Kit
NAP ELISA Kit
Lactose Broth
用于样品制备
POLY(VINYLSULFONIC ACID, SODIUM SALT)
OXANTEL PAMOATE
4-n-Octylbenzoic acid, 99%
2-Iodobenzyl alcohol, 99%
quinine sulphate
Disogluside
Solvent Black 34
3,3-Dimethylacrylic acid
3-CHLORO-2,6-DIMETHOXY-5-NITROBENZOIC ACID
Naphthalene
4-Azaindole
1,4-Bis(2-hydroxyhexafluoroisopropyl)benzene, 98%
(R)-(-)-1,3-Butanediol, 98%
1,2-Dichloropropane Standard
5-Fluoro-2-nitroanisole
Span 80
3-BroMo-5-nitrobenzaldehyde
4-(Propionyl)phenylboronic acid pinacol ester
7-Acetyl-5,8-dihydroxy-4-isopropyl-1-Methylbicyclo[4.3.0]nonane
Propionic acid hydrazide
2,4-Dichloro-5-(chloromethyl)pyrimidine
2-Ethyl-1-butanol, 98%
1-(methylsulfonyl)-1,4-diazepane(SALTDATA: HCl)
