C4-2B细胞：人前列腺癌复苏快细胞系 货号：GD-C4720506
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
体内细胞培养及其操作步骤：【瘤细胞悬液接种】1)无菌选取生长良好(有光泽，淡红色)瘤组织或对数生长期培养瘤细胞；2)在PBS中将瘤组织剪碎后用匀浆器研磨，经80～100目筛网过滤成细胞悬液；3)培养细胞应用PBS洗两遍；4)计数并调整细胞浓度至107～108／ml；5)常规消毒后，于接种部位(通常为背部或腋窝腹股沟皮下)用医用注射器皮下潜行一段后注入细胞悬液(0.1ml／部位，＞106细胞)。初次接种成功率低，细胞数尽可能多一些；6)次日注意观察动物一般情况。初次接种一般有一段较长的潜伏期，以后随着传代潜伏期逐渐缩短，固定为一个相对稳定的时间。【腹水瘤的建立与腹水瘤的接种】将实体瘤细胞直接种于小鼠腹腔、腹壁或其他部位，引起腹水，腹水中含有瘤细胞，将这种腹水反复传代，即可成为腹水瘤。初次传代时，腹水常呈血性(含大量红细胞)，反复传代后腹水逐渐变成乳白色。腹水瘤的接种过程如下。1)将冻存或培养的腹水瘤细胞离心和洗涤，进行细胞计数；2)消毒动物，左下腹穿刺接种106腹水瘤细胞；3)接种腹水瘤细胞后约7~12d，待小鼠腹部明显膨大。用碘酒棉球消毒小鼠腹部，用9号针头抽取腹水，也可行腹部解剖后，用滴管吸取。每只小鼠可抽3~5ml；4)抽取的腹水经3000rpm离心15min，收集上清，分装冻存备用。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
PalladiuM nitrate, Matrix Modifier Solution, Specpure|r
CloMiphene citrate salt
TRANS-3-HEXENEDIOIC ACID DIMETHYL ESTER
4-(METHYLTHIO)BENZONITRILE
7-Fluoro-2-Methylquinoline-8-boronic acid
Quinazoline
4-(Trifluoromethyl)benzoic anhydride
1-[3-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl]-1H-pyrazole , 97%
3'-Methoxybiphenyl-4-carboxaldehyde
3-Amino-4-bromo-1H-indazole, 95%
1H-Indazole-5-boronic acid, pinacol ester
2,6-Dichloropyrazine
MORPHOLIN-4-YL-PHENYL-ACETIC ACID
2-Chloro-4-nitrotoluene
(-)-1,3-Butanediol
3,4-Diaminofluorobenzene
Di-tert-butyl 3,3'-IMinodipropionate
Methyl 2,4-dibroMobutyrate
Methyl 3-hydroxyisoxazole-5-carboxylate, 99%
Tin AtoMic Absorption Standard
Isobutyl Isovalerate
4-(trifluoromethoxy)toluene
2-Chloropyridine-3-sulfonyl chloride
Zeolite
gamma-linolenic acid
LITHIUM METHOXIDE
3-Nitro-2-pyridinol
3-(Methylsulfinyl)phenylacetic acid, 96%
4-AMino-7-(trifluoroMethyl)quinoline, 98%
4-Hydroxy-3-MethoxybenzylaMine hydrochloride, 98%
Alloxan tetrahydrate
3,4-DiMethylbiphenyl-3-carboxylic acid
Diethyl ethylMalonate
N-NONADECANONITRILE
2-BroMo-1-[4-(Methylsulfonyl)phenyl]-1-ethanone
N,N'-Bis(3-Methylphenyl)-N,N'-bis(phenyl)benzidine
D-(+)-Raffinose Pentahydrate
Bromodifluoroacetic acid
3-(2,4-Dinitrophenylamino)propyltriethoxysilaneN-[3-triethoxysilylpropyl]-2,4 dinitrophenylamine
3-ethylmorpholine(SALTDATA: FREE)
1-BROMO-4-(TETRAFLUOROETHOXY)BENZENE
2-PHENYL-1-PROPANOL
Protected meropenem
DL-2-Phenylpropionic acid
2-Fluoro-4-(trifluoroMethyl)benzoyl chloride
1,3-Bis(3-aMinopropyl)tetraMethyldisiloxane [MonoMer for silicon Modified polyaMides]
Ethyl 7-hydroxypyrazolo[1,5-a]pyriMidine-6-carboxylate
AMberlite? CG-50 - type 1, ion-exchange resin
(3,3,3-Trifluoropropyl)chlorodiMethylsilane, 95%
1-BroMo-3-hexylbenzene
Span 85 (=Sorbitan Trioleate)
tert-Butyl peroxybenzoate
3-phenoxyazetidine hydrochloride
2-Cyano-4'-methylbiphenyl
2-(2-Aminothiazole-4-yl)-2-methoxyiminoacetic acid
1,2-BIS(DICYCLOHEXYLPHOSPHINO)ETHANE
Boc-iMinodiacetic acid
3-(3,5-Dichlorophenyl)propionic acid
2-(ChloroMethyl)allyl-triMethylsilane
4-methoxybutan-2-one(SALTDATA: FREE)
3-(Trifluoromethyl)benzylsulfonyl acetonitrile
DL-Phenylmercapturic Acid
2,6-Dimethyl-3,5-heptanedione
2-(TRIMETHYLACETYL)THIOPHENE
Rafoxanide
trans-4-Ethylcyclohexanecarboxylic acid
TRIETHANOLAMINE HYDROCHLORIDE
DL-Isoborneol
1-Decene
Moroxydine Hydrochloride
FMoc-d-ile-oh
5-BroMo-2-fluorobenzoic acid, 97%
1,2:5,6-Bis-O-(1-methylethylidene)-D-mannitol
Diosgenin
Ammonium benzenesulfonate
4-n-Octyloxyaniline
Rubber accelerator CED
taraxasterol acetate
GH ELISA Kit
Mouse ATPase (ATPase, Na+/K+ Transporting) ELISA Kit
Human FT4 ELISA Kit
Human OTOA ELISA Kit
IL-2R ELISA Kit
bTG ELISA Kit
Chicken adrenomedullin,ADM ELISA Kit
Human PKBG (Protein Kinase B Gamma) ELISA Kit
Human TSP-1 ELISA Kit
CENPH ELISA Kit
Slantz and Bartley medium
Enterococcus Broth
VinyloxytriMethylsilane, 97%
Furacilin
5-(2-Thienyl)-1H-pyrazole
2-Iodobutane, 99%, stab. with copper
4-(trifluoromethoxy)toluene
ZINC MESO-TETRAPHENYLPORPHINE
Solvent Yellow 79
amyl caproate
4'-Butyl-4-biphenylcarbonitrile
Sodium dichloroacetate
4-Nitrobenzaldehyde
2-Chloro-4-fluorobenzylaMine, 97%
N-(2-BroMophenyl)thiourea, 98%
N-Acetyl-3-O-β-D-galactopyranosyl-β-D-galactosaMine Methyl glycoside
4-Methylthiazole
Methyl 3-chloro-4-Methylbenzoate
DysprosiuM oxide
FMoc-Lys(Ddiv)-OH
4,5-DIPHENYL-2-METHYLTHIAZOLE
3-Chloropropiophenone 90%
(R)-(+)-2-(Methoxymethyl)-1-pyrrolidinecarboxaldehyde
2-Bromopyridine-3-boronic acid 96%
10,12-Tricosadiynoic Acid
