PageRuler TMPrestained Protein Ladder
RDM604 100μl Store at -20°C
Description
PageRuler TMPrestained Protein Ladder is a mixture of 10 recombinant，highly purified colored proteins with apparent molecular weight of 10kDa to 170kDa. Ladder proteins are covalently coupled with a blue dye except for two reference bands prestained with different colors .the 72KDa reference band is orange and 10KDa reference band is green.
The ladder is supplied in gel loading buffer and is ready-to-use; no heating , further dilution of a reducing agent required.
Contents
Approximately 0.1-0.2mg/ml of each protein in the storage buffer 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol)
Application
Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
Monitoring protein transfer onto membranes after Western Blotting.
Sizing of proteins on SDS-polyacrylamide gels and Western blots.
INSTRUCTION FOR USE
Thaw the Ladder at room temperature or heat at 37-40°C for a few minutes to dissolve precipitated solids. DO NOT BOIL!
Mix gently, but thoroughly to ensure the solution is homogeneous.
Load the Ladder on an SDS-PAGE gel and run.
Load volume:
5 µl per well for mini-gels,
10 µl per well for large gels,
After the run is complete, stain the gel or perform Western transfer procedure as desired.
Note
- Each lot of PageRuler™ Prestained Protein Ladder is calibrated against a precisely sized PageRuler™ Unstained Protein Ladder in a Tris-glycine gel and the calculated apparent molecular weights are reported in the product's Certificate of Analysis.
- Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system.
- A separation gel resolves proteins effectively according to their molecular weight. Linear gradient gels are used for resolution of both small and large proteins, while low percentage gels are recommended for analysis of large proteins. In these gels, small proteins migrate with the tracking dyes during electrophoresis.
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