| Polyclonal rabbit antibody | |
|---|---|
| Cat. No. 202 003 purified antibody | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20°C until use. |
| Applications | Blot: suitable for Dot Blot (1 : 1000 up to 1 : 10000; AP staining) blot IP: yes (see remarks) ICC: not tested yet IHC: not tested yet ELISA: suitable for sandwich-ELISA quantification |
| Immunogen | N6-methyladenosine fused to BSA. |
| Reactivity | Human, rat, mouse, Xenopus laevis, eukaryotes, prokaryotes. |
| Specificity | Specific for N6-methyladenosine (m6A). |
| Remarks | IP: Extracts from eukaryotic and prokaryotic cells. Standard protocol for the IP of nuclear extracts is provided with the product. For the isolation of m6A modified RNA from total or mRNA preparations the protocol according to Dominissini et al. (2013) is recommended. |
