| 分子量(MW): | 267.28 | |
|---|---|---|
| 化学式: | C16H13NO3 | |
| 溶解度: | DMSO ≥32mg/mL Water <1mg/mL Ethanol ≥3mg/mL | |
| 纯度: | >99% | |
| 稳定性: | at -20℃ 2 years | |
| CAS号: | 167869-21-8 |
PD 98059 is a highly selective inhibitor of MEK1 and MEK2 with IC50 values of 4 µM and 50 µM respectively[1–3] . PD98059 does not inhibit activation of other highly related dual-specificity protein kinases or the activity of over 18 other Ser/Thr protein kinases. At concentrations up to 100 µM, PD98059 does not inhibit activation of MKK3 or SEK (MKK4) as determined by measuring phosphorylation at its activation site. [1–3]
| T47D cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of PD98059 for 24 hours.
| |
| effect of ERK1/2 inhibitor PD98059 (1, 5, and 10 M) on SRP72 protein expression was evaluated on Jurkat cells stimulated with rhIL-1. The cells were harvested at 0,120, and 240 min, and the protein expression was verified byWBusing antibodies against human SRP72, human SRP54 (nonphosphorylated SRP protein), and humanGAPDHas constitutive protein.Adecreased SRP72 expression at 10M and 240 min was found.
| |
| C, effect of ERK1/2 inhibitor PD98059 (1, 5, and 10 M) was evaluated in SRP72-immunoprecipitated cell lysates. WB using anti-phosphoserine antibody showed a decreased SRP72 band when used as the inhibitor at concentrations of 1 M at 240 min, 5 M at 120 min, and 10 M at 120 and 240 min (lanes 3, 5, 8, and 9). D, results were analyzed and RUA illustrated, finding significant results at 1 M at 240 versus 0, 5 M at 120 versus 0, and 10 M at 120 versus 0 min.
| |
| We used real time RT-PCR to investigate the effects of PD98059 on SRP72 mRNA expression. All the samples were treated under the same experimental conditions used in protein tests done by WB, and phosphorylation was measured indirectly by IP-WB. The results did not show significant changes on the mRNA expression of SRP72 after the treatment with these inhibitors.
|
特定的存储和包装每个产品的信息在产品说明书上都有注明 。大多数Selleck产品，在推荐的条件下存储可稳定保存两年。产品有时建议的储存温度不同，大多数建议储存在-20 ° C ，抗体及蛋白等产品建议-60℃。YZ剂属于化学试剂，可在常温下运输储存两周左右。即使如此，我们保证产品的出货量将保持产品质量的条件下，一般都会放入冰袋。望阁下收到产品后，请按照产品数据表建议适当存储。
