基本信息
| 启动子: | ZB-actin promoter |
| 复制子: | pUC ori,f1 ori,SV40 ori |
| 质粒分类: | 哺乳细胞，转座子载体 |
| 质粒大小: | 7312bp |
| 原核抗性: | Kan |
| 筛选标记: | Neo |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | pEGFP-N-3:CGTCGCCGTCCAGCTCGACCAG |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
质粒图谱
质粒序列
LOCUS Exported 7312 bp ds-DNA circular SYN 07-DEC-2015
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTol2-Promoter-T2A-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7312)
AUTHORS hp
TITLE Direct Submission
JOURNAL Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
COMMENT http://www.informaxinc.com/
ORIGDB|GenBank
FEATURES Location/Qualifiers
source 1..7312
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 12..233
/note="Tol2 5'end"
promoter 262..2926
/note="ZB-actin promoter"
enhancer 2851..2870
/note="PF2Asunbio primer"
exon 2964..3044
/note="2A-peptide"
CDS 3051..3770
/codon_start=1
/note="EGFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 4017..4217
/note="Tol2 3'end"
rep_origin 4228..4683
/note="f1 ori"
rep_origin 5024..5159
/note="SV40 ori"
CDS 5208..6002
/codon_start=1
/note="Kan/Neo"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin 6587..7230
/note="pUC ori"
ORIGIN
1 tagttattaa tagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta
61 ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta
121 cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt
181 caaaaagtac ttattttttg gagatcactt cattctattt tcccttgcta ttagctagcg
241 ctaccggact cagatctcga gcgtttactc tgagaaactt ccatatttta gaggtaaaat
301 gctcagaatt gtaaaaagtc ttgtcaaaat acttggaatt tgccaaaaac aaaataaaag
361 tcacatcacc tcattgtcct tggtatcagc aagcactttt aacacaatca gctgtttttg
421 tttataactc ccactgaagg ccatgtgcat tactcccaag ttacacagta aaaagtcaca
481 tgtagtttat catgatcact gctgctcaag cacagcttta tctgttattc agtgtggcaa
541 tatctgaaat gtgtgcaaaa tattatttat tgtagctgat caccaacact ttcagtgttt
601 tttttttcta cttttaatga gaattatgtt cttaggattt atccttataa acctctttaa
661 aaagaaatgt tatgtctgca tagacacgac atgaaattat gaacagaaaa tttaactatt
721 taagatcaag acttgacata taacatttct aatgaacatt aaacctgagg taggcccact
781 tgcaaaaatg taactgctat cactcacttt atacatcatt gtgtaacttg tacacacaaa
841 aatagtgaag caatgtggca tggaatgcag gtcccattca ttcaaagtta ttaaagcact
901 ttcttaaagt gcattactac tgagctaaga ccagctagtt gctcatacaa catactacaa
961 gtagtgtcat cctaatttgg ataaaaaaaa tgtccatttt atacatggaa aagtactcat
1021 tagatccccc attgtttgta ttacggtatt tcgtgaacac aagaggtaaa tgacctacag
1081 agctgctgtt gtgttagatt gaaaacacaa cacaggatca tggaggcatc catcattatg
1141 acgcattctg ccacttgtaa ctctgcacag actttttgaa aagtttaatt gagtcttggc
1201 gttgtcgcag aaaaatacga aaaattctgt gttaaaattt gcaaataaaa attccagttt
1261 tataggaaat cgccatggcc ttgttcttta accagttaag cttccccttc tttcactctc
1321 aagttgcaag aagcaagtgt agcaatgtgc acgcgacagc cgggtgtgtg acgctggacc
1381 aatcagagca cagagctccg aaagtttacc ttttatggct agagccgggt atgtgccgtc
1441 atataaaaga gcgcgcccag cttttcagcc tcactttgag ctcctccaca cgcagctagt
1501 gcggaatatc atctgcttgt aacccattct cttaagtcga caacccccca aacccaaggt
1561 gagttggttt tacagttttt aagcgtttaa ttatagtttc tatctattaa tgttaagtaa
1621 aatgtgtaaa tggataaata tgatttgatg aagtctttag acagttcata acggtctctt
1681 tgtagttatc tactaggttt gatttataag tggtggttct tttgataatc tatgttacat
1741 ttaataaata ttggctgtat cttaactttt ttgtagcgtt agtggatgtg gcaggtgaga
1801 atgtcggtaa tttaacgtga ccagtcgtag gcacgacatt gaacattgaa taggccggtg
1861 tgaaatgggt gttaagtctt aatataaagt tgtgttctgg ctaatctctc tttaacagcc
1921 ataaatgtga tttttaacta cagttttgag ggtgctggtg agcatgttgc acacttgatg
1981 gatagccggc atgggaagtt ctttgtgcag gcagtgctgc agcagggtgt gacctacttt
2041 agctagccgg ctaaccagct ctcatctgat gtaacgtaaa ccccatcaat ctaacatcgg
2101 ctcattcgtg ctttataaga tcgaagatct tgttattagt aacgggtatt ggcatttagt
2161 tttaacttgg ttaattttct ttttgttatg tgttctattt aaatgtagcc tgcagccccc
2221 tctgtcttta atgaagctga agattaacgt taacgttagt gaagtgttgt atccgctatg
2281 aaagatttga attttagagg tgtggtccac tttaataacg gctgcggagt tctctggctg
2341 cacttcagcc gctgtgactg tcgaagcaga gcccatcagt ttttcaggat ctcggtgatt
2401 tttgaggcta gactatgaac tgaaccgact gagagtgacc gtccagaaat gctttagtgt
2461 gaagtctccg ctcacgagga gtccgtggac tgactgcaga tctgtgacgc agtaacaccc
2521 ggcggcagac acccgttgaa atgcggttgt gtaattgatc gcaggcgagg gtggaagagg
2581 atgtgaaact tcatttgtgt aaaatttagg gagtggtcct ggtgtgatga atgtcgaaat
2641 ctgttccttt ttactgagcc ctacgactct ggctgagtgc cacaccgccg gcagccgcaa
2701 agcggctcaa accattgcct tttatggtaa taatgagaga atgcagaggg acttcctttg
2761 tctggcatat ctgaggcgcg cgctgtcact agcgcccacc agcggtcaga ctgtagaatg
2821 cagagcaaaa caggaagttg actccagatg gtcacatgct tgctgaaacg cctctccctg
2881 gcagcagtgc aattctaaca gtttcccttt cttttacagt tcagccgaat tctgcagtcg
2941 acggtaccgc gggcccggga tccggaggtg gcagcggtgg cggtcagctg ttgaattttg
3001 accttcttaa acttgcggga gacgtcgagt ccaaccctgg gcccacaacc atggtgagca
3061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
3121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
3181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
3241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
3301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
3361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
3421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
3481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
3541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
3601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
3661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
3721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
3781 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
3841 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
3901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
3961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggctt
4021 aaacaagaat ctctagtttt ctttcttgct tttactttta cttccttaat actcaagtac
4081 aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac
4141 ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag
4201 tactttttac acctctgctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
4261 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
4321 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
4381 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
4441 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
4501 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
4561 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
4621 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
4681 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
4741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
4801 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
4861 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
4921 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
4981 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
5041 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
5101 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
5161 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
5221 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
5281 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
5341 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
5401 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
5461 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
5521 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
5581 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
5641 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
5701 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
5761 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
5821 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
5881 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
5941 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
6001 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
6061 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
6121 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
6181 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
6241 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
6301 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
6361 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
6421 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6721 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
7261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
温馨提示：不可用于临床ZL。
