ClonaCell™-HY Medium D半固体HAT杂交瘤细胞挑选培养基

品牌：stemcell technologies
型号：03804
产地：加拿大
供应商：北京诺为生物技术有限公司
供应商报价：询价
标签：ClonaCell™-HY Medium D半固体HAT杂交瘤细胞挑选培养基、ClonaCell™-HY Medium D半固体HAT杂交瘤细胞挑选培养基价格、ClonaCell™-HY Medium D半固体HAT杂交瘤细胞挑选培养基厂家、、、北京诺为生物技术有限公司

产品信息

供应商：北京诺为生物技术有限公司
保存条件： -20度储存

ClonaCell™-HY Medium D is a semi-solid methylcellulose-based medium containing the selective reagents hypoxanthine, aminopterin, and thymidine (HAT). This medium is used after fusion of splenocytes and myeloma cells to select and clone hybridomas in one step. Single cell-derived hybridomas stay together in the semi-solid medium as they grow to form monoclonal colonies. The hybridomas can easily be picked and screened, and then expanded for production of the desired antibody. This medium is suitable for use during development of monoclonal antibodies from a variety of host animals, including human, mouse, rat, hamster, and rabbit.

Benefits of semi-solid cloning:
• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated.
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution.
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium. As a result, rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures.

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