紫苏子油中α-亚麻酸的含量测定气相色谱仪

              xiangying  eagle analysis technology co., LTD. Set up one kind of determination of alpha linolenic acid in Perilla oil content determination method.

               Methods: GC7990 gas chromatograph, with hydrogen flame detector (FID). 30 m x 0.25 mm, 0.25 microns) for chromatographic column; Oven temperature: 215 ℃; Injection port temperature: 250 ℃; The detector temperature: 260 ℃. Nitrogen: 50 ml/min, spake shunt; Hydrogen: 40 ml/min. Air: 400 ml/min. Results: the quality of alpha linolenic acid concentration in 0.09028 ~ 0.68056 mg · mL - 1 (r = 0.9996) scope inside sex good, the average recovery was 99.57% (RSD = 0.67%, n = 9).

           Conclusion: using the oil sample (or sample extraction of fat), with potassium hydroxide saponification, methanol esterification in the presence of boron trifluoride, then gas chromatograph analysis, quantitative by internal standard method. The method is sensitive, accurate and reproducible, for the evaluation and control the quality of the Perilla seed oil to provide the basis.

           滕州市翔鹰分析技术有限公司建立1种测定紫苏子油中α-亚麻酸的含量测定方法。
           方法：GC7990气相色谱仪,附氢火焰（FID）检测器;30m×0.25mm,0.25μm）为色谱柱;柱箱温度：215℃;进样口温度：250℃;检测器温度：260℃;氮气：50mL/min,30：1分流;氢气：40mL/min;空气：400mL/min。结果：α-亚麻酸质量浓度在0.09028～0.68056mg·mL-1（r=0.9996）范围内线性关系良好,平均回收率为99.57%（RSD=0.67%,n=9）。
          结论：采用将油脂试样（或试样提取的脂肪）,经氢氧化钾皂化,甲醇酯化,然后用气相色谱仪分析,采用内标法定量。该方法灵敏、准确、重复性好,为评价和控制紫苏种子油的质量提供了依据。