Sera原装进口血清 胎 牛血清 德国原装进口

品Pai：	sera
CAS号：	S601P
供应商：	杭州昕诚生物科技有限公司
数量：	5000+
保质期：	6年
保存条件：	-20

胎 牛血清高端品PaiSeraPro由25年FBS生产经验的德国生产商SYSTECHGmbH（其产品占16%的份额）全程负责采血，过滤，生产，封装， 质量保证，，每瓶产品均可追溯品质来源，，其中SeraPro的胎牛血清（FBS）【商品号：S601P-500（胎牛血清），S601S- 500（标准血清）】适合绝大部分普通细胞培养和原代细胞的培养，例如：HEK293,MDBK, Hela, Vero, Sp2, K562, Raw264.7 等；，，针对干细胞培养，我们提供特制的血清，比如Pro ES（S742S-500）；针对贴壁细胞的培养：我们提供贴壁细胞专用血清：Pro AC（S732S-500）。
Lot No.: 1P1502
Date of production: November 20, 2014
Storage, stability, shipping:
Storage: -20° C
Stability: 6 years from date of production
Shipping: on dry ice
-------------------------------------------------------------------------------------------------------------------------------------------
Parameter Result Units
Appearance amber liquid n.a.
pH value 7.29 n.a.
Osmolality 316 mOsm/kg
Hemoglobin 17.3 mg/100 ml
Endotoxin 0.14 EU/ml
Total protein 41.65 mg/ml
Albumin 39.92 mg/ml
alpha-Globulin 0.47 mg/ml
beta-Globulin 2.26 mg/ml
IgG 221 µg/ml
Glucose 60.4 mg/100 ml
Cholesterol 45.4 mg/100 ml
Triglycerides 56.2 mg/100 ml
-------------------------------------------------------------------------------------------------------------------------------------------
Specification Result _
Sterility Incubation at 32° C sterile sterile
Incubation at 20° C sterile sterile
Mycoplasma not detected not detected
Virus testing
Bovine viral diarrhoea virus (BVDV) negative negative
Bovine herpes virus (BHV-1) negative negative
Para-influenza virus type 3 (PI-3) negative negative
Antibody testing
Bovine viral diarrhoea virus (BVDV) serological titer < 1:2
Bovine herpes virus (BHV-1) serological titer < 1:2
Para-influenza virus type 3 (PI-3) serological titer < 1:2
-------------------------------------------------------------------------------------------------------------------------------------------
Product Description Catalogue-No. Size
FBS Pro Fetal bovine serum, South America, Pro,
virus and mycoplasma tested S601P-500 500 ml
2
Performance (cell culture tested)
Cell growth (SP2/0-Ag14) Seed day 2 day 5 day 7 [cells per ml]
Lot no. 1P1502 1.00x10e3 1.00x10e4 8.25x10e5 1.19x10e6
Control serum 1.00x10e3 9.75x10e3 8.00x10e5 1.06x10e6
Cell growth (L929) [cells per ml]
Lot no. 1P1502 1.00x10e4 2.85x10e4 7.75x10e5 9.55x10e5
Control serum 1.00x10e4 2.45x10e4 7.20x10e5 8.90x10e5
No. of colonies/clones absolute % relative % _
Plating efficiency (L929)
Lot no. 1P1502 335 67 101
Control serum 331 66 100
Cloning efficiency (SP2/0-Ag14)
Lot. no. 1P1502 68 71 100
Control serum 68 71 100
TABLE 1:
Test Method
pH value Measured with pH-electrode
Osmolality Analyzed by freezing point depression
Hemoglobin Determined spectrophotometrically at three different wave lengths
Endotoxin Kinetic limulus amoebocyte lysate test (LAL)
Total protein Colorimetric test (Biuret reaction)
Albumin, Globulins Serum protein electrophoresis (SPEP)
IgG Radial immune diffusion
Glucose Colorimetric test (Trinder reaction)
Cholesterol Colorimetric test (CHOD-PAP)
Triglycerides Colorimetric test (Trinder reaction)
Sterility The absence of bacterial or fungal contamination is verified by dual incubation with Caso-Bouillon or ThioglycolatBouillon according to Ph. Eur. at 32°C and 20 °C
Mycoplasma Three different detection systems are used: DNA-binding fluorescence dye (DAPI), microscopic analysis of microbial
cultures and test kits which detect mycoplasma specific enzymes
Virus testing The following viruses and the presence of their antibodies are tested by cytopathic effect: Bovine viral diarrhoea virus
(BVDV), bovine herpes virus (BHV 1) and parainfluenza virus (PI-3)
Cell growth Growth test of murine myeloma cells (SP2/0-Ag14) and murine fibroblasts (L929)
Plating efficiency Murine fibroblasts (L929) are plated into a Petri dish, stained with Giemsa and after 14 days of incubation the fixed
cell colonies are counted (= absolute plating efficiency). The results are normalized to a previously tested reference
serum (= relative plating efficiency).
Cloning efficiency Murine myeloma cells (SP2/0-Ag14) are plated on microtiter plates (one cell per well). After 7 days of incubation the
developed cell colonies are counted (= absolute cloning efficiency). The results are normalized to a previously tested
reference serum (= relative cloning efficiency).
Suitability
FOR RESEARCH USE ONLY!
These products are intended for research or manufacturing use only.
Not for use in animal or human clinical or diagnostic application.
Raw material is collected in regularly inspected facilities and processed in compliance with current Ph. Eur. guidelines for Bovine
Sera.
Since raw serum is not pre-aged before filtration, turbidity or flocculent debris in form of precipitate may develop upon thawing or
storage of the product. This occurrence does not adversely affect the performance of the serum.
Results shown in this compilation have been obtained by carefully performing standard test methods (see table 1). Since results for
any specific test may vary depending on methodology, technical equipment, or test substances used, it is suggested that results for
particularly important parameters be repeated by the end user of this product.