Bovine SCO- spondin, SSPO ELISA KIT

Bovine SCO- spondin, SSPO ELISA KIT

Product Name:Bovine SCO- spondin, SSPO ELISA KIT
Packing:96T

Catalog No.:ELI-18705b

Gene Name:Bovine SCO-spondin, SSPO

Detect Range:0.781-50ng/ml

Sensitivity:0.469ng/ml

Target Protein Name:Bovine SCO-spondin, SSPO

Alternative Name:SSPO,Bovine SCO-spondin, SSPO
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Bovine SCO- spondin, SSPO ELISA KIT allows for the in vitro quantitative determination of Bovine SCO-spondin, SSPO concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Bovine SCO- spondin, SSPO ELISA KIT has been pre-coated with an Bovine SCO-spondin, SSPO antibody specific to Bovine SCO-spondin, SSPO .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine SCO-spondin, SSPO and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine SCO-spondin, SSPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine SCO-spondin, SSPO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

 

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