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GIBCO DMEM/F12 粉末培养基

产品信息
    • 品Pai/厂家:GIBCO
    • 产品名称:DMEM/F-12, powder, HEPES
    • 类型:培养基
    • 用途:科研
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    DescriptionDMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the .


    This DMEM/F-12 is modified as follows:

    With Without
    • L-glutamine • Sodium Bicarbonate
    • HEPES  
    • Phenol Red  

     


    The complete  is available.

    Using DMEM/F-12
    DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10%  (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see for details).

    cGMP manufacturing and quality system

    DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

     

     

    Specifications

    Glutamine:Phenol Red Indicator:Form:HEPES Buffer:Sodium Pyruvate Additive:Serum Supplementation:Glucose:Product Line:Product Size:Shipping Condition:
    L-Glutamine
    Phenol Red
    Powder
    HEPES
    Sodium Pyruvate
    Standard Serum Supplementation
    High Glucose
    Gibco®
    10 x 1 L
    Room Temperature

     

    Contents & storage

    Storage conditions: 2-8°C
    Shipping conditions: Ambient
    Shelf life: 36 months from date of manufacture

     

    Media formulations

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