The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors.
Product Name:CA IX/Carbonic Anhydrase IX Mouse Monoclonal Antibody
Clone Number: 11F9
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azideas Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:3,000 IP: 1:200
Optimal dilutions should be determined by the end user.
Specificity: CA IX Mouse Monoclonal antibody detects endogenous CA IX proteins.
Molecular Weight:35-58KD
Form: Liquid
Reactivity:H
Western blot analysis of 1) Hela, 2) 293T, with CA IX Mouse mAb diluted at 1:5,000.
1、Input: Hela Cell Lysate
2、IP product: IP dilute 1:200
Western blot analysis:primary antibody:BE31091:2,000
Secondary antibody: Goat anti-Mouse IgG,Light chain specific(BE0105),1:5,000
