大豆过敏原检测试剂盒（Soy ELISA Kit）

BioFront Technologies has developed the first comprehensive line of monoclonal antibody-based ELISA assay kits (MonoTrace ELISAs) for the detection of allergen protein contamination in a variety of food samples. MonoTrace ELISAs incorporate the use of a precisely defined set of handpicked monoclonal antibodies selected after a rigorous screening process for their ability to:

1、Target a major allergen resistant to food processing effects

2、Recognize a specific target, thereby eliminating cross-reactivity and reducing the possibility 

  of‘false positive’results

3、Perform well in a wide variety of food products with negligible food matrix interference

BioFront Technologies开发了条完全以单克隆抗体为基础的检测试剂盒产品（MonoTrace ELISAs），检测各种食物基质中的致敏蛋白污染。MonoTrace ELISAs综合使用一系列JZ确定的经过严格筛选过程后仔细挑选的单克隆抗体来实现：

1、分析耐食物加工的主要过敏原

2、特异性识别目标成分，从而消除交叉反应，以及减少“假阳性”结果的可能性

3、在各种食品（食品基质干扰可以忽略不计）的检测中表现优异

SOY ASSAY PERFORMANCE

大豆分析试剂盒性能

Overall Assay Time: Now just 30 minutes post-extraction!

Incubation Times: 10-minute steps for extraction, sample incubation, conjugate incubation, substrate incubation.

Number of Tests per Kit: 48/96

Limit of Detection: 0.14 ppm

Range of Quantification: 1 to 40 ppm

Specificity: Soy

*= calculations of parameters are based on representative data from ten assays, however, users should consult accompanying certificate of analysis for lot-specific values.

实验总时间：现在抽提后仅需30分钟！

孵育时间：抽提，样品孵育，二抗孵育，底物孵育每步仅需10分钟。

每一试剂盒测试数量：48/96

*检测限：0.14 ppm

*定量范围：1至40 ppm

特异性：大豆

*=参数的计算根据10次代表性的数据得出，然而，使用者应当查阅附随的分析证书中的具体批次数值。

CROSS-REACTIVITY 交叉反应

At concentrations as high as 100,000 ppm,  no cross-reactivity was observed in a large panel of assayed samples, including: walnut, hazelnut, pecan, peanut, pistachio, cashew, pecan, macadamia nut, Brazil nut, almond, pine nut, and sesame.

在浓度高达100,000 ppm时，对一份大型分析样品中没有观察到交叉反应，包括：核桃、榛子、胡桃、花生、开心果、腰果、澳洲坚果、巴西坚果、松子、芝麻。

RECOVERY 回收

Recovery of spiked soy samples at various concentrations was efficient (>79%) when the following food matrices were assayed: chocolate, cereals, breads, cake

当分析以下食物基质时，不同浓度大豆样品的加标回收率很高（>79%）：巧克力，谷类食品，面包，蛋糕。