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HCV NS3h Protein

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  • 丙型肝炎病毒NS3(解旋酶)蛋白片段

    Catalog Number: HCV-NS3h

    产品目录号:HCV-NS3h

    Genotype: 2a

    基因型:2a

    Form: Purified via Nickel affinity   chromatography

    形式:镍柱亲和层析纯化

    Tag: Expressed   with a C-terminal 6X His-tag

    标签:C6X   His-tag表达

    Origin:  E.coli

    来源:E.coli

    Applications: ELISAFlow   CytometrySDS-PAGEWestern   blot

    应用:酶联免疫吸附实验(ELISA)、流式细胞分析(Flow Cytometry)、SDS-PAGE、免疫印迹(Western blot

    Buffer/Composition: 1X PBS pH 7.2; 1X protease inhibitor   cocktail, 1 mM PMSF

    缓冲液/成分:1X PBS pH 7.21X   protease inhibitor cocktail1 mM PMSF

    Volume: 50μl/ 250μl/ 500μl

    体积:50μl/ 250μl/ 500μl

    Concentration: 2 μg/μl

    浓度:2 μg/μl

    Storage:  Short Term: 4    Long Term (up to 1 year): -20(avoid   repeated freeze/thaw)

    储存:短期:4    长期(可达1年):-20℃(避免反复冻融)

     

    Coomassie stained gel showing purification of recombinant NS3 helicase protein.Bacterial cells expressing the His-tagged protein were lysed (lane 2), pelleted, and the SN (lane 3) was loaded onto Nickel resin. The ~55 kD protein was absent in the resin flow thru (lane 4) as well as column washes (lanes 5 and 6). Elutions with immidazole (lanes 7 and 8) yielded ≥95% pure protein.Lane 1 = marker


    考染显示纯化的重组NH3解旋酶蛋白。表达His标签蛋白的菌细胞裂解物(lane 2),颗粒状的SNlane 3)过镍树脂。~55 kD蛋白在树脂流出液((lane 4)中消失,同见于柱洗涤液(lanes 5 & 6)。洗脱液连同咪唑(lanes 7 & 8)产生≥95%纯度的蛋白质。Lane 1 = marker


     

    • Item #: HCV-NS3h & HCV-NS3h-500 & HCV-NS3h-1

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