CD2 Antibody (11-0029-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human/Non-Human Primate CD2 FITC (right). Cells in the lymphocyte gate were used for analysis.产品细节已验证应用 稀释倍数 流式细胞分析 (Flow) 5 µL (0.25 µg)/test 已发表应用 流式细胞分析 (Flow) 查看下方已发表的2篇文献 免疫组化（冰冻） (IHC (F)) 查看下方已发表的1篇文献 产品规格 种属反应 Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Pig, Rhesus monkey 已发表种属 Human 宿主/亚型 Mouse / IgG1, kappa 推荐的同型对照 Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ 分类 Monoclonal 类型 Antibody 克隆号 RPA-2.10 偶联物 FITC 查看其它类型 激发/发射光谱 查看光谱 形式 Liquid 浓度 5 µL/Test 纯化类型 Affinity chromatography 保存液 PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA 内含物 0.09% sodium azide 保存条件 4° C, store in dark, DO NOT FREEZE! RRID AB_10718390
产品详细信息
Description: The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.
RPA-2.10 crossreacts to non-human primates and pigs.
Applications Reported: The RPA-2.10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells. CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. The interaction betwee CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection. Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.
Peripheral monocytes are functionally altered and invade the CNS in ALS patients.
"11002942 was used in flow cytometry to find that central nervous system infiltration of peripheral monocytes correlates with improved motor neuron survival in a genetic amyotrophic lateral sclerosis mouse model"
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cell adhesion molecule 
