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Nutridoma-SP罗氏Roche货号11011375001

产品信息
  • Application

    For the serum-free cultivation of murine myelomas and hybridomas that have an intact cholesterol biosynthesis pathway, such as those derived from the SP2/0, and some of the P3x63Ag8.653 cell lines and lymphoblastoid cell lines, as well as for primary lymphoid cell cultures. It is also used for the serum-free culture of a variety of other cell types, including neural explants.

    Product Description

    Biological activity: Assayed for its ability to support the growth of the SP2/0 myeloma cell line.
    Working concentration: Nutridoma-SP concentrate is diluted 1:100 (v/v) in basal medium. We recommend the use of high glucose DMEM/ RPMI 1640 (1:1). The protein concentration is less than 50 µg/ml at the working concentration.

    Background Information

    Nutridoma is a family of chemically defined supplements that can completely replace serum in cell culture media. Nutridoma-SP is used for the cultivation of established myeloma and hybridoma cells.

    Contents

    Solution, 100x concentrated, filtered through 0.2 μm pore size membrane

    Biochemically defined serum-free medium supplement composed of albumin, insulin, transferrin, and other defined organic and inorganic compounds. Nutridoma contains no other growth factors, mitogens, hormones, or sterols.
    The solution contains the pH indicator phenol red and appears clear.

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