Rat F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F37142 Catalog Size Price LS-F37142-1 1 plate $990 USD Request Bulk Add to Wish List Add to CartPerformance Guaranteed SpecificationsPublicationsReviewsImagesPopular F2 / Prothrombin / Thrombin Elisa Kits Bovine F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13468 Reactivity: Bovine Range: 7.8-500 ng/ml Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13466 Reactivity: Human Range: 0.78-50 ng/ml Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F22981 Reactivity: Human Range: 15.63-1000 pg/ml Human F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F4268 Reactivity: Human Range: 4.69-300 ng/ml Rat F2 / Prothrombin / Thrombin ELISA Kit (Sandwich ELISA) - LS-F13467 Reactivity: Rat Range: 1.56-100 ng/mlProduct DescriptionLS-F37142 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat F2 / Prothrombin / Thrombin in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of F2 / Prothrombin / Thrombin as low as 0.094 nanograms per millilter. More F2 / Prothrombin / Thrombin Products Top of PageSpecificationsType
F2, Prothrombin B-chain, Coagulation factor II, FACTOR II, Prothrombin, PT, RPRGL2, Serine protease, THPH1, Thrombin Reactivity
Rat Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
0.156 - 10 ng/ml Sensitivity
0.094 ng/ml Precision
Intra-Assay: CV<8% Inter-Assay: CV<10% Storage
Store at 4°C. Stable for 6 months. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- F? antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-F? antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the F? amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of F? can be calculated. Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About F2 / Prothrombin / Thrombin
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Finally, peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. P00734 NM_000506 NP_000497.1
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