体外细菌分离试剂盒 （大肠杆菌和其他革兰氏阴性菌）

Overview

The only kit for easy isolation of bacterial outer membrane vesicles (OMVs)

Expanding our exosome expertise to bacteria, SBI now offers the ExoBacteria^TM OMV Isolation Kit. The first and only kit designed to streamline isolation of bacterial outer membrane vesicles (OMVs), the ExoBacteria OMV Isolation Kit uses an innovative, precipitation-free gravity column system to harvest OMVs from bacterial culture medium. With OMVs in less than 1-hour and delivering a purity and yield rivaling ultracentrifugation-based approaches, the ExoBacteria OMV Isolation Kit is a great way to accelerate your studies on bacterial communication and pathogenesis¹, cancer therapy², bacterial modulation of the host immune response³, and bacterial OMV engineering for use as vaccines⁴.

Transmission electron micrograph (TEM) of OMVs isolated using the ExoBacteria OMV Isolation Kit (light blue arrows).

• Unique—the only dedicated isolation system for bacterial OMVs
• Fast—go from cultured bacterial media to purified OMV in less than 1 hour, twice as fast as ultracentrifugation
• Simple—easy-to-setup, precipitation-free gravity column system
• Better—get significantly higher yields and purity compared to ultracentrifugation-based approaches

Each kit comes with enough reagents and consumables to process up to 20 reactions*, enabling maximal flexibility with experimental conditions.

*1 reaction is defined as 30 ml of purified bacterial culture media loaded into a single gravity column.

 

REFERENCES

1. Vanaja SK, et al. Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation. Cell 2016 May 19; 165(5): 1106-1119.
2. Kim OY, et al. Bacterial outer membrane vesicles suppress tumor by interferon-γ-mediated antitumor response. Nat Commun. 2017 Sep 20;8(1):626. doi: 10.1038/s41467-017-00729-8
3. Vidakovics ML, et al. B cell activation by outer membrane vesicles–a novel virulence mechanism. PLoS Pathog. 2010 Jan 15;6(1):e1000724. doi: 10.1371/journal.ppat.1000724.
4. Chen DJ, et al. Delivery of foreign antigens by engineered outer membrane vesicle vaccines. Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3099-104. doi: 10.1073/pnas.0805532107. Epub 2010 Jan 27.

 

How It Works

Demystifying OMV Isolation: A newer, faster, simpler method with high yields and purity

1. Clarify up to 30 ml of bacteria culture media
2. Pour supernatant into ExoBacteria OMV isolation column
3. Wash column with 15 ml of binding buffer
4. Elute bacterial OMVs with 1.5 ml of elution buffer