abcam发育生物学兔单克隆抗体Anti-160 kD Neurofilament Medium (phospho S614 + S619)抗体[EPR580(2)Y]

• 描述
  兔单克隆抗体[EPR580(2)Y] to 160 kD Neurofilament Medium (phospho S614 + S619)
• 经测试应用WB, IHC-Pmore details
• 种属反应性
  与反应: Human
• 免疫原

  A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human 160 kD Neurofilament Medium

• 阳性对照
  • SH-SY5Y cell lysates; human brain tissue
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• 纯度Tissue culture supernatant
• 克隆单克隆
• 克隆编号EPR580(2)Y
• 同种型IgG
• 研究领域
  • Neuroscience
  •  
  • Neurology process
  •  
  • Neurodegenerative disease
  • Alzheimer's disease
  •  
  • Other

  • Neuroscience
  •  
  • Cell Adhesion Proteins
  •  
  • Cytoskeletal Proteins
  • Intermediate Filaments

  • Neuroscience
  •  
  • Cell Type Marker
  •  
  • Neuron marker
  •  
  • Soma marker

  • Signal Transduction
  •  
  • Cytoskeleton / ECM
  •  
  • Cytoskeleton
  • Intermediate Filaments
  •  
  • Class IV
  •  
  • Neurofilaments

  • Stem Cells
  •  
  • Lineage Markers
  •  
  • Ectoderm

  • Developmental Biology
  •  
  • Lineage specification
  •  
  • Ectoderm

应用

• 应用说明IHC-P: 1/100 - 1/250. 
  WB: 1/500. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa). 
  
  Is unsuitable for Flow Cyt, ICC or IP. 
  
  
  Not yet tested in other applications. 
  Optimal dilutions/concentrations should be determined by the end user.

靶标

• 相关性Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
• 细胞定位Cytoplasm
• 数据库链接
  • Entrez Gene: 4741 Human
  • Omim: 162250 Human
  • SwissProt: P07197 Human
  • Unigene: 458657 Human
• 别名
  • 150kDa medium antibody
  • NEF3 antibody
  • NEFM antibody

  see all

Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] 图像

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
  Immunohistochemical analysis of paraffin-embedded human brain tissue using ab68142 at a dilution of 1/100 - 1/250
• Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142)
  All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] (ab68142) at 1/500 dilution
  
  Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase
  Lane 2 : Untreated SH-SY5Y cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP conjugated Goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 102 kDa
  Observed band size : 160 kDa (why is the actual band size different from the predicted?)