Collagenase IV 胶原酶IV Sigma C5138

其他说明

Analysis Note

Contains clostripain, neutral protease and tryptic activities.

Unit Definition

One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA Hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One Neutral Protease Unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.

TEST

SPECIFICATION

appearance

report result

collagen digestion activity

greater than 125 units/mg solid

unit definition

One unit liberates peptides from collagen (C9879) equivalent in ninhydrin color to 1.0 micromole of leucine in 5 hours at pH7.4 at 37degc in the presence of calcium ions.

falgpa hydrolysis activity

0.5 to 5.0 units/mg solid

unit definition

One unit hydrolyzes 1.0 micromole of falgpa (furylacryloyl-leu-gly-pro-ala, F5135) per minute at pH7.5 at 25degc in the presence of calcium ions.

caseinase activity

report result

clostripain activity

report result

suitability test

Suitable for the preparation of hepatocytes from rat liver.

shelf life

13 years

document #

c5138/01/27/06/7