细胞凋亡甲基化芯片

Human Apoptosis DNA Methylation PCR Array, Signature Panel:  EAHS-121Z
The Human Apoptosis EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 promoters of genes involved in apoptosis, or programmed cell death. Dysregulation of apoptosis, leading to uncontrolled cell growth, is a hallmark of carcinogenesis. Hypermethylation of pro-apoptotic genes (BNIP3L) silences their expression, which can lead to tumor progression. Profiling cellular or fresh tissue genomic DNA samples with these arrays may help correlate CpG island methylation status with biological phenotypes. The results may also help provide further insights into the role dysregulated apoptosis plays in diseases such as cancer. With a simple restriction enzyme digestion and real-time PCR, research studies can analyze the promoter methylation status of 22 different apoptosis genes with this DNA methylation PCR array. Both 96-well and 384-well ( 4 X 96 ) formats are available. The EpiTect Methyl II PCR Arrays use the MethylScreen™ Technology provided under license from Orion Genomics, LLC.

Induction of Apoptosis: BAD, BAX, BCL2L11, BCLAF1, BID, BIK, BNIP3L, CASP3, CIDEB, CRADD, DAPK1, DFFA, FADD, GADD4, HRK, LTBR, TNFRSF21, TNFRSF25 (DR3), TP53.
Anti-Apoptosis: BAX, BNIP3L, DAPK1, HRK.
Regulation of Apoptosis:
Negative Regulation: BIRC2, BNIP3L, CASP3, DAPK1, DFFA, TP53.
Positive Regulation: BAD, BAX, BCL2L11, BCLAF1, BID, BIK, BNIP3L, CIDEB, CRADD, FADD, HRK, TNFRSF25 (DR3), TP53.
Caspases & Regulators: APAF1, BAX, CASP3, CASP9, CRADD, TP53.