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GenMute™ siRNA Transfection Reagent for Neuro2A Cell

产品信息
  • Description:
    GenMute™ siRNA Transfection Reagent for Neuro-2A Cell is pre-optimized for transfecting siRNA to Neuro-2A cells.Refer to the following optimal transfection conditions for maximal silencing on Neuro-2A cells. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.

    Summary of Optimal Transfection Conditions:
    Cell culture medium
    Confluency of cell on the day of transfection
    Optimal siRNA concentration
    Diluent for siRNA and GenMute
     Reagent
    Incubation Time to Form GenMute™/siRNA Complex
    Maximal Silencing Efficiency 


    Transfection Results:
    Reporter Gene
    Silencing Efficiency (% )


    DMEM with 4.5 g/L glucose, 10% FBS
    ~50%
    30 nM

    1xGenMute™ Transfection Buffer
    ~15 minutes at RT
    48 hours


    Renilla Luciferase
    90%


    Storage:
    Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

    Data Sheet & Protocol .pdf
    温馨提示:不可用于临床ZL。
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