小鼠HA单克隆抗体

 

Mouse Monoclonal Antibody to HA-tag

 

General Information
Immunogen:	HA peptide conjugated with KLH
Clone ID:	Mouse mAb
Ig Type:	IgG1
Applications:	WB , IHC , IP
Specificity:	Specifically detected HA tag fused protein.
Formulation:	0.2µm filtered solution in PBS with 50％ glycerol
Storage:	<-20℃

 

Preparation

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with peptide YPYDVPDYA conjugated with KLH. The ascites was purified by ion exchange chromatography.

Applications

1) Western blot (1:1000) : This antibody can be used at 2μg with appropriate secondary reagents to detect HA-tagged proteins.

 

 

M  1  2  3  4

Lane 1, 2: 293T control Lane 3, 4: 293T transfected with HA plasmid M: protein ladder

kDa

 

Immunoprecipitation (1:500)：This antibody can be used at 2μg to precipitate HA-tagged proteins.

 

Lane 1: HA mAb IP with 293T transfected with HA plasmid Lane2: HA mAb IP with 293T transfected without HA plasmid M: protein ladder

M 1 2

 

 

 

 

 

Immunofluorescence (1:100): This antibody can be used at 2μg to detect HA-tagged proteins.

 

 

 

 

 

 

 

 

 

 

 

293T transfected with HA plasmid

 

 

 

 

Specificity

Mouse mAb specifically detects over-expressed proteins containing the HA epitope tag.

Storage

Store at -20℃, and stable for one year from the date of shipment.